Early delivery of human umbilical cord mesenchymal stem cells improves healing in a rat model of Achilles tendinopathy

被引：2
作者：
Yuan, Ze [1 ]
Yu, Ding [2 ]
Wang, Yanxue [1 ]
Liu, Lijiaqi [1 ]
Wang, Junchao [1 ]
Ma, Chao [1 ]
Wu, Shaoling [1 ]
机构：
[1] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Rehabil Med, Guangzhou, Peoples R China
[2] South China Univ Technol, Guangzhou Peoples Hosp 1, Sch Med, Dept Rehabil Med, Guangzhou, Peoples R China
来源：
REGENERATIVE MEDICINE | 2024年 / 19卷 / 02期
关键词：
Achilles tendinopathy; collagen fiber; delivery time; mesenchymal stem cells; PLATELET-RICH PLASMA; TENDON REPAIR; EARLY-STAGE;
D O I：
10.2217/rme-2023-0222
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Objective: This study aimed to explore the efficacy and optimal delivery time of human umbilical cord mesenchymal stem cells (hUC-MSCs) in treating collagenase-induced Achilles tendinopathy. Methods: Achilles tendinopathy in rats at early or advanced stages was induced by injecting collagenase I into bilateral Achilles tendons. A total of 28 injured rats were injected with a hUC-MSC solution or normal saline into bilateral tendons twice and sampled after 4 weeks for histological staining, gene expression analysis, transmission electron microscope assay and biomechanical testing analysis. Results: The results revealed better histological performance and a larger collagen fiber diameter in the MSC group. mRNA expression of TNF-alpha, IL-1 beta and MMP-3 was lower after MSC transplantation. Early MSC delivery promoted collagen I and TIMP-3 synthesis, and strengthened tendon toughness. Conclusion: hUC-MSCs demonstrated a therapeutic effect in treating collagenase-induced Achilles tendinopathy, particularly in the early stage of tendinopathy.
引用
收藏
页码：93 / 102
页数：10
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