On-tissue chemical derivatization-enhanced spatially resolved lipidomics reveals abnormal metabolism in type 2 diabetic rat brain

被引：3
作者：
Wang, Lingzhi [1 ]
Zang, Qingce [1 ]
Tu, Xinyi [1 ]
Li, Xinzhu [1 ]
Li, Ting [1 ]
Zhu, Shiyu [1 ]
Zhang, Ruiping [1 ,2 ]
Abliz, Zeper [1 ,2 ,3 ]
机构：
[1] Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Bioact Subst & Funct Nat Med, Inst Mat Med, Beijing 100050, Peoples R China
[2] Minzu Univ China, Key Lab Mass Spectrometry Imaging & Metabol, Natl Ethn Affairs Commiss, Beijing 100081, Peoples R China
[3] Minzu Univ China, Coll Life & Environm Sci, Ctr Imaging & Syst Biol, Beijing 100081, Peoples R China
来源：
TALANTA | 2024年 / 269卷
关键词：
Mass spectrometry imaging; Diabetes; On-tissue chemical derivatization; Brain; Spatially resolved lipidomics; ACID;
D O I：
10.1016/j.talanta.2023.125491
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Neurologic disorders are often accompanied by alterations in lipids and oxylipins in the brain. However, the complexity of the lipidome in the brain and its changes during brain damage caused by diabetes remain poorly understood. Herein, we developed an enhanced spatially resolved lipidomics approach with the assistance of ontissue chemical derivatization to study lipid metabolism in the rat brain. This method enabled the spatially resolved analysis of 560 lipids and oxylipins in 19 brain microregions in coronal and sagittal sections and remarkably improved the coverage of lipidome detection. We applied this method to lipidomic studies of the diabetic rat brain and found that lipid dysregulation followed a microregion-specific pattern. Carnitines and glycerolipids were mainly elevated in the corpus callosum (midbrain) and pineal gland regions, respectively. In addition, most oxylipins, including fatty aldehydes and oxo fatty acids, were significantly upregulated in nine brain microregions. We produced a spatially resolved analysis of lipids and oxylipins, providing a novel analytical tool for brain metabolism research.
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页数：9
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