Proximity hybridization regulated dual-mode ratiometric biosensor for estriol detection in pregnancy serum

被引：8
作者：
Jiao, Yan [1 ]
Li, Hongyuan [2 ,3 ]
Wang, Huan [4 ]
Feng, Qiumei [4 ]
Gao, Yongguang [2 ]
机构：
[1] Xuzhou Cent Hosp, Dept Obstet & Gynecol, 199 Jiefang Rd, Xuzhou 221009, Jiangsu, Peoples R China
[2] Xuzhou Cent Hosp, Dept Radiol, 199 Jiefang Rd, Xuzhou, Jiangsu, Peoples R China
[3] Xuzhou Cent Hosp, Dept Neurol, 199 Jiefang Rd, Xuzhou 221009, Jiangsu, Peoples R China
[4] Jiangsu Normal Univ, Sch Chem & Mat Sci, Xuzhou 221116, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2023年 / 1278卷
关键词：
Estriol; Dual-mode biosensor; Ratiometric detection; Proximity hybridization; Resonance energy transfer; RESONANCE ENERGY-TRANSFER; ELECTROCHEMILUMINESCENCE; STRATEGY; SENSOR;
D O I：
10.1016/j.aca.2023.341689
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Sensitive and accurate determination of estriol level is vastly significant for the fetal growth and development. Herein, we constructed a dual-mode ratiometric biosensor for estriol assay combining the competitive immunoreaction, proximity hybridization with a two-step resonance energy transfer (RET) strategy. Estriol antibody and goat anti-rabbit antibody labeled DNA probes (Ab1-DNA1-Pt NPs and Ab2-DNA2) both hybridized with silver nanoclusters labeled DNA strands (H1-Ag NCs). Thus, the formed proximity hybridization enabled the occurrence of fluorescence RET (FL-RET, as the primary RET) between Ag NCs (donor) and Pt NPs (acceptor), quenching FL intensity of Ag NCs (FL off). When target estriol existed, the competitive reaction of Ab1-DNA1-Pt NPs with estriol and Ab2-DNA2 avoided the proximity hybridization. Then, the estriol-dependent H1-Ag NCs quenched electrochemiluminescence (ECL) emission of CdS quantum dots (CdS QDs, ECL off), generating ECLRET (as the second RET). Consequently, according to the reverse changes of FL and ECL responses, this SENSOR realized the quantification of estriol from 1 to 100 ng/mL. Moreover, satisfactory results were achieved while testing estriol in pregnancy serum specimens, suggesting that the system is promising for potential application in samples analysis.
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页数：8
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