Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support

被引：0

作者：

Kolesnichenko, Alena V. ^{[1
]}

Kazmina, Nathalia A. ^{[1
]}

Chistov, Alexey A. ^{[1
]}

Vakhrenev, Roman G. ^{[1
]}

Kolesanova, Ekaterina F. ^{[1
]}

机构：

[1] Inst Biomed Chem, 10-8 Pogodinskaya Ul, Moscow 119121, Russia

来源：

AMINO ACIDS | 2023年 / 55卷 / 09期

关键词：

Peptide-p-nitroanilides; Oxone(& REG; Desalting; Bio-Beads(& REG; ) SM-2; Adsorption chromatography; Phenyl group; SOLID-PHASE SYNTHESIS; PROTEASE; STRATEGY; REMOVAL; ASSAY;

D O I：

10.1007/s00726-023-03302-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polystyrene-based support Bio-Beads((R)) SM-2 was employed for desalting peptide-p-nitroanilides from Oxone((R)). Neither tosyl, 9-fluorenyl(methoxycarbonyl), p-nitroanilide groups nor indolyl or p-hydroxyphenyl side-chains of Trp and Tyr ensured an efficient adsorption of peptide-p-nitroanilides onto Bio-Beads((R)) SM-2. Only unsubstituted phenyl-containing protection groups (carbobenzoxy or benzoyl) and Phe residues provided the adsorption of peptides on Bio-Beads((R)) SM-2 and their efficient desalting. This support is well suitable for multiple parallel phenyl group-containing peptide derivative separations and high-throughput screenings.

引用

页码：1137 / 1140

页数：4

共 7 条

[1] Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support
Alena V. Kolesnichenko
Nathalia A. Kazmina
Alexey A. Chistov
Roman G. Vakhrenev
Ekaterina F. Kolesanova
Amino Acids, 2023, 55 : 1137 - 1140
[2] Peptide design using ω-amino acids:: Unusual turn structures nucleated by an n-terminal single γ-aminobutyric acid residue in short model peptides
Maji, SK
Banerjee, R
Velmurugan, D
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Banerjee, A
JOURNAL OF ORGANIC CHEMISTRY, 2002, 67 (03): : 633 - 639
[3] INFLUENCE OF MOLECULAR-STRUCTURE ON HALF-LIFE AND HYDROLYSIS OF DIPEPTIDES IN PLASMA - IMPORTANCE OF GLYCINE AS N-TERMINAL AMINO-ACID RESIDUE
ADIBI, SA
PALEOS, GA
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[4] Reactions of gold(III) complexes withl-histidine-containing dipeptides: influence of chelated ligand and N-terminal amino acid on the rate of peptide coordination
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Stanojevic, Ivana M.
Andrejevic, Tina P.
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[5] Protection efficacy of the Brucella abortus ghost vaccine candidate lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36) in murine models
Kwon, Ae Jeong
Moon, Ja Young
Kim, Won Kyong
Kim, Suk
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JOURNAL OF VETERINARY MEDICAL SCIENCE, 2016, 78 (10): : 1541 - 1548
[6] Induction of one-handed helical screw sense in achiral peptide through the domino effect based on interacting its N-terminal amino group with chiral carboxylic acid
Inai, Y
Tagawa, K
Takasu, A
Hirabayashi, T
Oshikawa, T
Yamashita, M
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (47) : 11731 - 11732
[7] The N-terminal lysine residue-rich domain II and the 340-430 amino acid segment of eukaryotic initiation factor 2-associated glycoprotein p67 are the binding sites for the γ-subunit of eIF2
Ghosh, Arnab
Datta, Rekha
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EXPERIMENTAL CELL RESEARCH, 2006, 312 (16) : 3184 - 3203

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