LINC00664/miR-411-5p/KLF9 feedback loop contributes to the human oral squamous cell carcinoma progression

被引:9
|
作者
Wang, Chengyong [1 ]
Wang, Qinglian [2 ]
Weng, Zuquan [3 ]
机构
[1] Fujian Med Univ, Union Hosp, Dept Oral & Maxillofacial Surg, Fuzhou 350001, Peoples R China
[2] Fudan Univ, Zhongshan Hosp, Xiamen Branch, Dept Stomatol, Xiamen, Peoples R China
[3] Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou, Peoples R China
关键词
KLF9; LINC00664; miR-411-5p; oral squamous cell carcinoma; LONG NONCODING RNA; CANCER; PROLIFERATION;
D O I
10.1111/odi.14033
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives Oral squamous cell carcinoma (OSCC) is one of the most aggressive head and neck cancers with high incidence. Multiple studies have revealed that long non-coding RNAs (lncRNAs) play pivotal roles in tumorigenesis. However, the role of long intergenic non-protein coding RNA 664 (LINC00664) on the progression of OSCC was still unclear. Subjects and methods In this study, the expression of LINC00664 in OSCC tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The functional role of LINC0664 was estimated by cell counting kit-8 (CCK-8), transwell assays, Western blot in vitro, and xenograft tumor model in vivo. The regulatory mechanism was investigated by RNA-binding protein immunoprecipitation (RIP), chromatin immunoprecipitation (ChIP), and luciferase reporter assays. Results LINC00664 was found to be upregulated in OSCC tissues and cell lines and was associated with poor prognosis of OSCC patients. LINC00664 knockdown suppressed OSCC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). Moreover, Kruppel like factor 9 (KLF9) enhanced LINC00664 expression at transcription level. Interestingly, LINC00664 upregulated KLF9 expression by sponging miR-411-5p. In addition, knockdown of LINC00664 restrained tumor growth of OSCC in vivo. Conclusion Our study identified the oncogenic roles of LINC00664 in OSCC tumorigenesis and EMT via KLF9/LINC00664/miR-411-5p/KLF9 feedback loop, which provides new perspectives of the potential therapeutic target for OSCC.
引用
收藏
页码:672 / 685
页数:14
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