Combined immunohistochemical protocols to differentiate macrophages within the mononuclear-phagocyte system

被引：2

作者：

Lazzarini, G. ^{[1
]}

Abramo, F. ^{[1
]}

Albanese, F. ^{[2
]}

Pirone, A. ^{[1
]}

Miragliotta, V. ^{[1
]}

机构：

[1] Univ Pisa, Dept Vet Sci, Viale Piagge 2, I-56124 Pisa, Italy

[2] Vet Diagnost Lab MyLav La Vallonea, Passirana Di Rho, MI, Italy

来源：

ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER | 2023年 / 249卷

关键词：

Mononuclear phagocyte system; Skin; Macrophages; Histocytes; Dendritic cells; DENDRITIC CELLS; MONOCYTES; DOGS;

D O I：

10.1016/j.aanat.2023.152107

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Background: The "mononuclear phagocyte system" (MPS) refers to dispersed mononuclear monocytes and macrophages and is used to distinguish them from polymorphonuclear cells. The term "histiocyte" indicates large cells with voluminous granulated cytoplasm, sometimes containing engulfed particles, recognized as fully differentiated end cells of the MPS. Dendritic cells (DC) represent another diversified population whose inclusion in the MPS is still debated. The diverse cells of the MPS cannot all be characterized by single antigen markers or unique functions expressed at all stages of cell differentiation or activation. Nevertheless, in a diagnostic setting, their reliable identification plays a major role when a specific therapy must be established. Understanding the heterogeneity among MPS cell populations is indeed relevant to define different therapeutic approaches that can range from the use of antibiotics to immunomodulatory agents. For this reason, we attempted to establish a protocol to reliably identify the proportion of macrophages within the mononuclear phagocyte system in a tissue and/or in a given inflammatory population. Methods: the Tafuri method was used in different double immunofluorescence protocols using an anti-Iba1, anti-MAC387, and anti-CD11b-CD68-CD163-CD14-CD16 antibody. Results and discussion: in normal canine skin the anti-Iba-1 antibody stained an epidermal cell population (i.e. Langerhans cells) and scattered cells within the dermal compartment. MAC387 was unable to stain cells containing Leishmania amastigotes in leishmaniasis-diagnosed samples as the anti-CD11b-CD68-CD163CD14-CD16 antibody did. By using a combination of staining protocols to differentiate macrophages within the whole histiocytic infiltrate we validated the use of a cocktail of rabbit monoclonal antibodies raised against CD11b, CD68, CD163, CD14, CD16 to stain skin macrophages. & COPY; 2023 Elsevier GmbH. All rights reserved.

引用

页数：6

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