A rapid method for sex identification in Cannabis sativa using high resolution melt analysis

被引：2
|
作者：
Gilchrist, Erin J. [1 ]
Hegebarth, Daniela [1 ]
Wang, Shumin [1 ]
Quilichini, Teagen D. [1 ]
Sawler, Jason [1 ]
Toh, Shir Yi [1 ]
Foley, Cody [1 ]
Page, Jonathan E. [1 ]
机构：
[1] Anandia Labs, Vancouver, BC V5T 4T5, Canada
来源：
BOTANY | 2023年 / 101卷 / 07期
关键词：
Cannabis sativa L; hemp; Humulus lupulus L; hops; sex identification; sex-specific SNPs; high resolution melt analysis; CHROMOSOMES; EXPRESSION; PLANTS;
D O I：
10.1139/cjb-2021-0168
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
We report the identification of two single nucleotide polymorphisms in Cannabis sativa L. that are associated with female and male plant sex phenotypes and are located on the top arm of the X chromosome. High resolution melt analysis was used to develop and validate a novel, rapid method for sex identification in medical and (or) recreational cannabis as well as in hemp. This method can distinguish between dioecious male (XY) and dioecious female (XX) cannabis plants with 100% accuracy and can also be used to differentiate between male and female Humulus lupulus L. (hop) plants.
引用
收藏
页码：284 / 290
页数：7
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