Synthesis, Bioactivity Evaluation, and Cell Imaging of Isorhamine Zwitterionic Polymers

Using the four hydroxyl reaction points of isorhamnetin to introduce zwitterionic groups, five kinds of acid ionic polymer derivatives were prepared. Their ultraviolet spectrum (UV) and fluorescence spectra (PL) were 332 and 405 nm, respectively, in different solvents. In order to observe the dynamic self-assembly effect of the amphiphilic polymer drugs more directly, it was found that the amphiphilic polymer drugs aggregated together in a circular or elliptical shape under a transmission electron microscope (TEM), and was wrapped with isorhamnetin. In terms of biocompatibility, imaging experiments were performed using HeLa cells at different densities of 200 and 400 to observe the targeting changes. In MTT assay, isorhamnetin poly(carboxybetaine methacrylate)-1 (I) and isorhamnetin poly(carboxybetaine methacrylate)-2 (II) were particularly significant, and their activities in HCT-116 and HeLa were (53.27 +/- 1.17, 68.51 +/- 4.55), and (55.11 +/- 5.87, 67.62 +/- 6.28), respectively. Finally, in the cell electron microscope environment, it can be intuitively found that isorhamnetin amphoteric polymer drugs can directly enter both the targeted liposomes of HeLa cells and even enter the nucleus.