Asgard ESCRT-III and VPS4 reveal conserved chromatin binding properties of the ESCRT machinery

被引:5
作者
Nachmias, Dikla [1 ,2 ]
Melnikov, Nataly [1 ,2 ]
Zorea, Alvah [1 ,2 ]
Sharon, Maya [1 ,2 ]
Yemini, Reut [1 ,2 ]
De-picchoto, Yasmin [1 ,2 ]
Tsirkas, Ioannis [1 ,2 ]
Aharoni, Amir [1 ,2 ]
Frohn, Bela [3 ]
Schwille, Petra [3 ]
Zarivach, Raz [1 ,4 ]
Mizrahi, Itzhak [1 ,2 ]
Elia, Natalie [1 ,2 ]
机构
[1] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
[2] Ben Gurion Univ Negev, Natl Inst Biotechnol Negev NIBN, IL-84105 Beer Sheva, Israel
[3] Max Planck Inst Biochem, Dept Cellular & Mol Biophys, D-82152 Martinsried, Germany
[4] Ben Gurion Univ Negev, Ilse Katz Inst Nanoscale Sci & Technol, IL-84105 Beer Sheva, Israel
基金
以色列科学基金会; 欧洲研究理事会;
关键词
MEMBRANE SCISSION; CELL-DIVISION; PROTEIN; ARCHAEA; PSPA;
D O I
10.1038/s41396-022-01328-2
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The archaeal Asgard superphylum currently stands as the most promising prokaryotic candidate, from which eukaryotic cells emerged. This unique superphylum encodes for eukaryotic signature proteins (ESP) that could shed light on the origin of eukaryotes, but the properties and function of these proteins is largely unresolved. Here, we set to understand the function of an Asgard archaeal protein family, namely the ESCRT machinery, that is conserved across all domains of life and executes basic cellular eukaryotic functions, including membrane constriction during cell division. We find that ESCRT proteins encoded in Loki archaea, express in mammalian and yeast cells, and that the Loki ESCRT-III protein, CHMP4-7, resides in the eukaryotic nucleus in both organisms. Moreover, Loki ESCRT-III proteins associated with chromatin, recruited their AAA-ATPase VPS4 counterpart to organize in discrete foci in the mammalian nucleus, and directly bind DNA. The human ESCRT-III protein, CHMP1B, exhibited similar nuclear properties and recruited both human and Asgard VPS4s to nuclear foci, indicating interspecies interactions. Mutation analysis revealed a role for the N terminal region of ESCRT-III in mediating these phenotypes in both human and Asgard ESCRTs. These findings suggest that ESCRT proteins hold chromatin binding properties that were highly preserved through the billion years of evolution separating Asgard archaea and humans. The conserved chromatin binding properties of the ESCRT membrane remodeling machinery, reported here, may have important implications for the origin of eukaryogenesis.
引用
收藏
页码:117 / 129
页数:13
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