An efficient and reproducible Agrobacterium-mediated genetic transformation method for the ornamental monocotyledonous plant Ornithogalum dubium Houtt

被引：4

作者：

Tripathi, Pankaj Kumar ^{[1
]}

Ayzenshtat, Dana ^{[1
]}

Kumar, Manoj ^{[1
]}

Zemach, Hanita ^{[1
]}

Yedidia, Iris ^{[1
]}

Bocobza, Samuel E. E. ^{[1
]}

机构：

[1] ARO Volcani Ctr, Inst Plant Sci, POB 15159, IL-7528809 Rishon Leziyyon, Israel

来源：

PLANT GROWTH REGULATION | 2023年 / 101卷 / 01期

关键词：

Agrobacterium; Bulbous ornamentals; Monocotyledonous geophyte; Ornithogalum dubium; Plant transformation; UBIQUITIN10; promoter; PARTICLE BOMBARDMENT; REGENERATION; EXPRESSION; CALLUS; RNA;

D O I：

10.1007/s10725-023-01013-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ornithogalum is a genus from the Hyacinthaceae (Asparagaceae) family that comprises about 200 species with remarkable white, yellow, or orange flowers that display exceptional vase life. These properties have made it a popular cut flower and pot plant. Forward genetics approaches may be advantageous to generate novel phenotypes, but the Agrobacterium-mediated transformation of plants from this genus remains challenging. Here, a stable and efficient Agrobacterium-mediated transformation system was established for O. dubium. We found that the timing of transformation with respect to light exposure of the tissue affected transformation rates more than other tested parameters. In the transgenic plants obtained, T-DNA integrations were confirmed by polymerase chain reactions and positive plants were established in the greenhouse and displayed weak transgene expression. This study exposed an efficient platform for gene function research and germplasm improvement in O. dubium plants. The present protocol is now available for the development of novel improved O. dubium varieties.

引用

页码：201 / 214

页数：14

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