Regeneration of Humeral Head Using a 3D Bioprinted Anisotropic Scaffold with Dual Modulation of Endochondral Ossification

被引：11

作者：

Li, Tao ^{[1
,2
]}

Ma, Zhengjiang ^{[1
]}

Zhang, Yuxin ^{[3
]}

Yang, Zezheng ^{[4
]}

Li, Wentao ^{[1
]}

Lu, Dezhi ^{[5
]}

Liu, Yihao ^{[1
]}

Qiang, Lei ^{[6
]}

Wang, Tianchang ^{[1
]}

Ren, Ya ^{[6
]}

Wang, Wenhao ^{[6
]}

He, Hongtao ^{[7
]}

Zhou, Xiaojun ^{[8
]}

Mao, Yuanqing ^{[1
]}

Zhu, Junfeng ^{[2
]}

Wang, Jinwu ^{[1
]}

Chen, Xiaodong ^{[2
]}

Dai, Kerong ^{[1
]}

机构：

[1] Affiliated Shanghai Jiao Tong Univ, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implant, Shanghai Ninth Peoples Hosp,Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China

[2] Shanghai Jiao Tong Univ, Dept Orthopaed, Xinhua Hosp, Sch Med, 1665 Kongjiang Rd, Shanghai 200092, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Coll Stomatol,Dept Oral Surg,Natl Ctr Stomatol,Nat, Shanghai 200011, Peoples R China

[4] Fudan Univ, Peoples Hosp Shanghai 5, Dept Orthoped, Shanghai 200240, Peoples R China

[5] Shanghai Univ, Sch Med, Shanghai 200444, Peoples R China

[6] Southwest JiaoTong Univ, Coll Med, 111 North 1st Sect Second Ring Rd, Chengdu 610036, Peoples R China

[7] Dalian Med Univ, Ward Dept Orthoped 3, Hosp 2, 467, Zhongshan Rd, Dalian 116000, Liaoning Provin, Peoples R China

[8] Donghua Univ, Coll Biol Sci & Med Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China

来源：

ADVANCED SCIENCE | 2023年 / 10卷 / 12期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

3D bioprinting; biomechanical stimuli; dynamic compression; endochondral ossification; humeral joint; MESENCHYMAL STEM-CELLS; CHONDROGENIC DIFFERENTIATION; OSTEOGENIC DIFFERENTIATION; BONE REGENERATION; STROMAL CELLS; CARTILAGE; JOINT; TRANSPLANTATION; VASCULARIZATION; HYPERTROPHY;

D O I：

10.1002/advs.202205059

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tissue engineering is theoretically thought to be a promising method for the reconstruction of biological joints, and thus, offers a potential treatment alternative for advanced osteoarthritis. However, to date, no significant progress is made in the regeneration of large biological joints. In the current study, a biomimetic scaffold for rabbit humeral head regeneration consisting of heterogeneous porous architecture, various bioinks, and different hard supporting materials in the cartilage and bone regions is designed and fabricated in one step using 3D bioprinting technology. Furthermore, orchestrated dynamic mechanical stimulus combined with different biochemical cues (parathyroid hormone [PTH] and chemical component hydroxyapatite [HA] in the outer and inner region, respectively) are used for dual regulation of endochondral ossification. Specifically, dynamic mechanical stimulus combined with growth factor PTH in the outer region inhibits endochondral ossification and results in cartilage regeneration, whereas dynamic mechanical stimulus combined with HA in the inner region promotes endochondral ossification and results in efficient subchondral bone regeneration. The strategy established in this study with the dual modulation of endochondral ossification for 3D bioprinted anisotropic scaffolds represents a versatile and scalable approach for repairing large joints.

引用

页数：17

共 76 条

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