A Label-free Electrochemical Immunosensor Based on Gold Nanoparticles-poly(ferriporphyrin-co-acrylamide)-reduced Graphene Oxide and the Application in Prostate Specific Antigen Detection

被引：3

作者：

Han, Bingkai ^{[1
,2
]}

Chen, Yuan ^{[2
]}

Wang, Haotian ^{[1
]}

Zhao, Wenying ^{[1
]}

Hu, Yuhan ^{[1
]}

Guo, Yayun ^{[1
]}

Yan, Jilong ^{[1
]}

Jia, Shixiong ^{[1
]}

机构：

[1] Tianjin Univ Sport, Inst Sport Exercise & Hlth, Tianjin Key Lab Exercise Physiol & Sports Med, 16 Donghai Rd,West Tuanbo New Town, Tianjin 301617, Peoples R China

[2] Nankai Univ, Coll Life Sci, Key Lab Bioact Mat, Minist Educ, Weijin Rd 94, Tianjin 300071, Peoples R China

来源：

ELECTROCHEMISTRY | 2024年 / 92卷 / 02期

关键词：

Prostate Specific Antigen; Ferriporphyrin; Nanocomposite; Biosensor; BREAST-CANCER; NANOPARTICLES; DIAGNOSIS; ELECTROCATALYSIS; NANOCOMPOSITES; CHITOSAN; HEMATIN; CA-19-9; FILM; CEA;

D O I：

10.5796/electrochemistry.23-00104

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Prostate specific antigen (PSA), as a biomarker, plays important roles in early diagnosis of male prostate diseases, especially prostate cancer. In this study, a novel electrochemical probe polymer of poly(ferriporphyrin-co-acrylamide) was synthesized, by further combined with reduced graphene oxide and gold nanoparticles, and oriented immobilization of PSA antibody, a label -free electrochemical immunosensor was obtained, and then successfully applied in the determination of PSA in 10% non -antigen serum system. The prepared biosensor expressed high selectivity toward PSA, with a LOD of 0.001 ng mL-1, a linear range of 0.01-110 ng mL-1, and a sensitivity of 15.78 mu A mL ng-1. What's more, it showed good stability, well repeatability and high selectivity in the real PSA sample detection. This work provided certain references for the design of the new electrochemical probes and the detection of tumor markers.

引用

页数：9

共 39 条

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