Simultaneous pharmacokinetic assessment of phytopharmaceuticals in fenugreek extract using LC-MS/MS in Sprague-Dawley rats

被引：19

作者：

Agrawal, Sristi ^{[1
,2
]}

Bisen, Amol Chhatrapati ^{[1
,2
]}

Biswas, Arpon ^{[1
,3
]}

Sanap, Sachin Nashik ^{[1
,2
]}

Verma, Sarvesh Kumar ^{[1
,3
]}

Kumar, Mukesh ^{[1
,3
]}

Jaiswal, Shubham ^{[2
,4
]}

Kumar, Ashok ^{[4
]}

Narender, Tadigoppula ^{[4
]}

Bhatta, Rabi Sankar ^{[1
,2
,5
]}

机构：

[1] CSIR, Cent Drug Res Inst, Pharmaceut & Pharmacokinet Div, Lucknow, India

[2] Acad Sci & Innovat Res AcSIR, Ghaziabad, India

[3] Jawaharlal Nehru Univ, New Delhi, India

[4] CSIR, Cent Drug Res Inst, Div Med & Proc Chem, Lucknow, India

[5] CSIR, Cent Drug Res Inst, Pharmaceut & Pharmacokinet Div, BS 10-1,Sect 10,Jankipuram Extens,Sitapur Rd, Lucknow 226031, India

来源：

BIOMEDICAL CHROMATOGRAPHY | 2023年 / 37卷 / 05期

关键词：

4-hydroxyisoleucine; LC-MS; MS; pinitol; polycystic ovary syndrome; raffinose; Trigonella foenum-graecum; trigonelline; 4-HYDROXYISOLEUCINE;

D O I：

10.1002/bmc.5600

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fenugreek seeds are used in numerous marketed herbal formulations with therapeutic benefits. Some of its bioactive components such as 4-hydroxyisoleucine, trigonelline, raffinose, and pinitol are reported to possess potential therapeutic activities, such as antibacterial, antidiabetic, stomach stimulant, and anti-invasive, against hyperandrogenism and other allied diseases, including polycystic ovary syndrome. A fully validated, selective, and sensitive bioanalytical method for the simultaneous rapid quantification of the aforementioned bioactive components has been developed using hyphenated liquid chromatography electrospray tandem mass spectrometry. The analytes were separated within 5 min using gradient elution in a C18 column at a flow rate of 0.5 ml/min. Plasma protein precipitation technique was employed to isolate the analytes from the samples. Oral pharmacokinetic profile of the four bioactive components in Sprague-Dawley rats was further evaluated using noncompartmental analysis using Phoenix WinNonlin software.

引用

页数：12

共 31 条

[1]

Anandakumar P., 2018, Advanced Journal of Pharmacie and Life Science Research, V6, P1

[2]

Avalos-Soriano A., 2016, RESISTANCE ASS OBESI, V6, P1, DOI [10.3390/molecules21111596, DOI 10.3390/MOLECULES21111596]

[3] Validated high-performance thin-layer chromatography method for determination of trigonelline in herbal extract and pharmaceutical dosage form [J].