Evaluation of the antibacterial activity of hecogenin acetate and its inhibitory potential of NorA and MepA efflux pumps from Staphylococcus aureus

被引：9

作者：

Araujo, Nara Juliana Santos ^{[1
,2
]}

Silva, Ana Raquel Pereira da ^{[3
,4
]}

Costa, Maria do Socorro ^{[3
,4
]}

Silva, Camila Aparecida Pereira ^{[2
]}

de Freitas, Thiago Sampaio ^{[4
]}

de Sousa, Erlanio Oliveira ^{[5
]}

Barbosa Filho, Jose Maria ^{[6
]}

Matos, Yedda Maria Lobo Soares de ^{[4
]}

Coutinho, Henrique Douglas Melo ^{[4
]}

Andrade-Pinheiro, Jacqueline Cosmo ^{[1
,2
]}

机构：

[1] Fed Univ Cariri, Grad Program Hlth Sci, BR-63180000 Barbalha, CE, Brazil

[2] Fed Univ Cariri, R Olegario Emidio Araujo, s-n, Brejo St, BR-63260000 Brejo Sant, CE, Brazil

[3] Univ Estadual Ceara, Grad Program Biotechnol, Fortaleza, CE, Brazil

[4] Reg Univ Cariri, Lab Microbiol & Mol Biol, LMBM, Crato, CE, Brazil

[5] Fac Technol Cariri, FATEC, Juazeiro do Norte, CE, Brazil

[6] Univ Fed Paraiba, Lab Technol Pharmaceut, Joao Pessoa, PB, Brazil

来源：

MICROBIAL PATHOGENESIS | 2023年 / 174卷

关键词：

Saponin; Resistance; Efflux inhibitor; ANTIOXIDANT;

D O I：

10.1016/j.micpath.2022.105925

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antimicrobial drugs are of great importance in the control of bacterial infections. Its indiscriminate use con-tributes to the consolidation of bacterial resistance. Its applicability is due to its secondary metabolites, such as saponins, which are compounds with relevant antibacterial action. Hecogenin acetate is a saponin present in plants of the agave genus with analgesic, antioxidant, antinociceptive, cardioactive, anticancer, antifungal and antimicrobial activity. The present work aimed to identify the antibacterial activity of hecogenin acetate against strains of E. coli, P. aeruginosa and S. aureus and to investigate the NorA and MepA efflux pump inhibitory activity of S. aureus strains. The Minimum Inhibitory Concentration was evaluated by broth microdilution. The Antibiotic Activity Modifier effect and the assessment of efflux pump inhibition were evaluated by microdilution with sub -inhibitory concentrations. Hecogenin acetate showed minimal inhibitory concentration without significant relevance. In the evaluation of the potentiating activity of the antibiotic action, a greater antagonistic behavior is noticed. In the analyzes performed with the efflux pump, it was noticed that the hecogenin acetate does not interfere in the efflux pump mechanism of the analyzed bacteria.

引用

页数：6

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