1-Methylcyclopropene Pretreatment in ?La France? Pears to Extend Postharvest Life and Maximize Fruit Quality

被引：4

作者：

Kazimi, Abdul H. ^{[1
]}

Mitalo, Oscar W. ^{[1
,2
]}

Azimi, Azimullah ^{[1
]}

Masuda, Kanae ^{[1
]}

Yano, Chikara ^{[1
]}

Akagi, Takashi ^{[1
]}

Ushijima, Koichiro ^{[1
]}

Kubo, Yasutaka ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Environm & Life Sci, Okayama 7008530, Japan

[2] Univ Tsukuba, Fac Life & Environm Sci, Tsukuba 3058572, Japan

来源：

HORTICULTURE JOURNAL | 2023年 / 92卷 / 02期

基金：

日本学术振兴会;

关键词：

ethylene; softening capacity; RNA-Seq; softening; storage life; PACKAGED BARTLETT PEARS; RIPENING PROCESS; SHELF-LIFE; ETHYLENE; 1-MCP; STORAGE; TEMPERATURE; EXPRESSION; GENES; TIME;

D O I：

10.2503/hortj.QH-012

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

A major challenge in terms of commercializing 1-methylclopropene (1-MCP) to extend the storage life and control physiological disorders in European pears is that it irreversibly inhibits fruit ripening in some cultivars, particularly flesh softening that is necessary for optimal consumption quality. In this study, we examined the effect of 1-MCP pretreatments on fruit ripening and associated transcriptomic changes in 'La France' (Pyrus communis L.) pears during storage at 20 degrees C and 5 degrees C. Compared to non-treated controls, 1-MCP pretreatment suppressed fruit respiration and ethylene production rates, and markedly delayed flesh softening. Normal ripening (ethylene production and flesh softening to eating quality firmness) was observed in 1-MCP treated fruit after 42 d at 20 degrees C, and 112 d at 5 degrees C. Subsequent RNA-Seq analysis revealed that 6,427 genes, including those associated with ethylene biosynthesis (ACS1, ACS1b, ACO1, and ACO2), cell wall degrading enzymes (PG3, fl-GAL, EG, and EXP1), and transcription factors (AGL18 and NAC29) were up -or down-regulated in non-treated fruit both at 20 degrees C and 5 degrees C. The expression patterns of these genes were disrupted by 1-MCP pretreatment, but up-or down-regulation was also observed when ethylene was detected in 1-MCP-treated fruit. Together, these findings demonstrate the potential for practical use of 1-MCP to extend storage life in 'La France' pears given that (i) a single application markedly extended storage life to 56 d at 20 degrees C and 112 d at 5 degrees C, and (ii) treated fruit could regain their softening capacity, thus eliminating previous irreversible ripening blockage concerns.

引用

页码：105 / 114

页数：10

共 43 条

[21] Pseudo-chromosome-length genome assembly of a double haploid "Bartlett" pear (Pyrus communis L.) [J].

Linsmith, Gareth ;

Rombauts, Stephane ;

Montanari, Sara ;

Deng, Cecilia H. ;

Celton, Jean-Marc ;

Guerif, Philippe ;

Liu, Chang ;

Lohaus, Rolf ;

Zurn, Jason D. ;

Cestaro, Alessandro ;

Bassil, Nahla V. ;

Bakker, Linda V. ;

Schijlen, Elio ;

Gardiner, Susan E. ;

Lespinasse, Yves ;

Durel, Charles-Eric ;

Velasco, Riccardo ;

Neale, David B. ;

Chagne, David ;

Van de Peer, Yves ;

Troggio, Michela ;

Bianco, Luca .

GIGASCIENCE, 2019, 8 (12)

[22] Ethylene Control of Fruit Ripening: Revisiting the Complex Network of Transcriptional Regulation [J].

Liu, Mingchun ;

Pirrello, Julien ;

Chervin, Christian ;

Roustan, Jean-Paul ;

Bouzayen, Mondher .

PLANT PHYSIOLOGY, 2015, 169 (04) :2380-2390

[23] Enhancement of fruit shelf life by suppressing N-glycan processing enzymes [J].

Meli, Vijaykumar S. ;

Ghosh, Sumit ;

Prabha, T. N. ;

Chakraborty, Niranjan ;

Chakraborty, Subhra ;

Datta, Asis .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (06) :2413-2418

[24] Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene [J].

Mitalo, Oscar W. ;

Otsuki, Takumi ;

Okada, Rui ;

Obitsu, Saeka ;

Masuda, Kanae ;

Hojo, Yuko ;

Matsuura, Takakazu ;

Mori, Izumi C. ;

Abe, Daigo ;

Asiche, William O. ;

Akagi, Takashi ;

Kubo, Yasutaka ;

Ushijima, Koichiro .

JOURNAL OF EXPERIMENTAL BOTANY, 2020, 71 (16) :4778-4796

[25] 'Passe Crassane' pear fruit (Pyrus communis L.) ripening: Revisiting the role of low temperature via integrated physiological and transcriptome analysis [J].

Mitalo, Oscar W. ;

Tosab, Yasuaki ;

Tokiwa, Sumire ;

Kondo, Yuki ;

Azimi, Azimullah ;

Hojo, Yuko ;

Matsuura, Takakazu ;

Mori, Izumi C. ;

Nakano, Ryohei ;

Akagi, Takashi ;

Ushijima, Koichiro ;

Kubo, Yasutaka .

POSTHARVEST BIOLOGY AND TECHNOLOGY, 2019, 158

[26] Low Temperature Storage Stimulates Fruit Softening and Sugar Accumulation Without Ethylene and Aroma Volatile Production in Kiwifruit [J].

Mitalo, Oscar W. ;

Tokiwa, Sumire ;

Kondo, Yuki ;

Otsuki, Takumi ;

Galis, Ivan ;

Suezawa, Katsuhiko ;

Kataoka, Ikuo ;

Doan, Anh T. ;

Nakano, Ryohei ;

Ushijima, Koichiro ;

Kubo, Yasutaka .

FRONTIERS IN PLANT SCIENCE, 2019, 10

[27] Combined signal sequence trap and macroarray analysis identifies genes associated with differential fruit softening characteristics during ripening in European and Chinese pears [J].

Mwaniki, Mercy W. ;

Mitalo, Oscar W. ;

Mworia, Eric G. ;

Owino, Willis O. ;

Hiwasa-Tanase, Kyoko ;

Rose, Jocelyn K. C. ;

Aoki, Koh ;

Esumi, Tomoya ;

Kawai, Takashi ;

Nakano, Ryohei ;

Ushijima, Koichiro ;

Kubo, Yasutaka .

POSTHARVEST BIOLOGY AND TECHNOLOGY, 2021, 174

[28] Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis 'Sanuki Gold' [J].

Mworia, Eric G. ;

Yoshikawa, Takashi ;

Yokotani, Naoki ;

Fukuda, Tetsuo ;

Suezawa, Katsuhiko ;

Ushijima, Koichiro ;

Nakano, Ryohei ;

Kubo, Yasutaka .

POSTHARVEST BIOLOGY AND TECHNOLOGY, 2010, 55 (02) :108-113

[29] 'Bartlett' pear fruit (Pyrus communis L.) ripening regulation by low temperatures involves genes associated with jasmonic acid, cold response, and transcription factors [J].

Nham, Ngoc T. ;

Macnish, Andrew J. ;

Zakharov, Florence ;

Mitcham, Elizabeth J. .

PLANT SCIENCE, 2017, 260 :8-18

[30] Time-resolved and continuous wave NIR reflectance spectroscopy to predict soluble solids content and firmness of pear [J].

Nicolai, Bart M. ;

Verlinden, Bert E. ;

Desmet, Michele ;

Saevels, Stijn ;

Saeys, Wouter ;

Theron, Karen ;

Cubeddu, Rinaldo ;

Pifferi, Antonio ;

Torricelli, Alessandro .

POSTHARVEST BIOLOGY AND TECHNOLOGY, 2008, 47 (01) :68-74

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