A novel fluorescent probe for rapid detection of sulfatase in vitro and in living cells

被引：5

作者：

Chen, Junqing ^{[1
]}

Peng, Zihao ^{[1
]}

Ji, Min ^{[2
]}

Wang, Peng ^{[3
]}

机构：

[1] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China

[2] Southeast Univ, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China

[3] China Pharmaceut Univ, Sch Engn, Nanjing 210009, Peoples R China

来源：

BIOORGANIC CHEMISTRY | 2023年 / 138卷

基金：

中国国家自然科学基金;

关键词：

Sulfatase; Fluorescent probe; Quinoline-malononitrile; Imaging; STEROID SULFATASE; ARYLSULFATASE; INHIBITORS; ENZYME;

D O I：

10.1016/j.bioorg.2023.106655

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sulfatase participates in a variety of physiological processes in organisms including hormone regulation, cell signaling, and bacterial pathogenesis. Current sulfatase fluorescent probes can be used to track sulfate esterase overexpression in cancer cells for diagnostic purposes and to understand the pathological activity of sulfate esterase. However, some sulfatase fluorescent probes based on the hydrolysis of the sulfate bond were easily disturbed by the catalytic activity of sulfatase. Herein, we developed the fluorescent probe BQM-NH2 for sulfatase detection, which was based on the quinoline-malononitrile. The probe BQM-NH2 showed a fast response to sulfatase within 1 min and satisfactory sensitivity with a calculated LOD of 1.73 U/L. Importantly, it was successfully used to monitor endogenous sulfate in tumor cells, indicating BQM-NH2 has the potential to monitor sulfatase under physiological and pathological conditions.

引用

页数：6