Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish

被引：0

作者：

Alanazi, Fehaid ^{[2
]}

Elderdery, Abozer Y. ^{[1
]}

Alzahrani, Badr ^{[1
]}

Alzerwi, Nasser A. N. ^{[3
]}

Althobiti, Maryam Musleh ^{[4
]}

Rayzah, Musaed ^{[3
]}

Alanazi, Abdulaziz Suailem ^{[5
]}

Kuriri, Fahd A. ^{[4
]}

Idrees, Bandar ^{[6
]}

Alenazy, Fawaz O. ^{[1
]}

Alsultan, Afnan ^{[7
]}

Rayzah, Fares ^{[8
]}

Baksh, Yaser ^{[9
]}

Subbiah, Suresh Kumar ^{[10
]}

Mok, Pooi Ling ^{[11
]}

机构：

[1] Jouf Univ, Coll Appl Med Sci, Dept Clin Lab Sci, Sakaka, Saudi Arabia

[2] Jouf Univ, Coll Appl Med Sci AlQurayyat, Dept Clin Lab Sci, Sakaka, Saudi Arabia

[3] Majmaah Univ, Coll Med, Dept Surg, POB 66, Riyadh 11952, Saudi Arabia

[4] Shaqra Univ, Coll Appl Med Sci, Dept Clin Lab Sci, Shaqra 15572, Riyadh Province, Saudi Arabia

[5] King Fahad Med City, Pathol & Clin Lab Med Adm Flow Cytometry, Riyadh, Saudi Arabia

[6] Prince Sultan Mil Med City Riyadh, Dept Surg, Makkah Al Mukarramah Rd, As Sulimaniyah, Saudi Arabia

[7] King Saud Med City, Dept Surg, Riyadh 12746, Saudi Arabia

[8] Aseer Cent Hosp, Dept Surg, Abha, Saudi Arabia

[9] Iman Gen Hosp, Dept Surg, Riyadh 12684, Saudi Arabia

[10] Bharath Inst Higher Educ & Res, Ctr Mat Engn & Regenerat Med, Chennai, India

[11] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Biomed Sci, Upm Serdang 43400, Selangor, Malaysia

来源：

E-POLYMERS | 2023年 / 23卷 / 01期

关键词：

Pluronic F127; chlorogenic acid nanoparticles; HepG2 cell lines; PI3K/AKT; mTOR pathways; OXIDATIVE STRESS; NANOCARRIER; INDUCTION;

D O I：

10.1515/epoly-2023-0053

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

In this study, chlorogenic acid nanoparticles encapsulated in Pluronic F127 polymer were synthesized and characterized to determine if they could treat human liver cancer. The nanoparticles were synthesized using standard procedures and characterized using physical and biological techniques such as X-ray diffraction, Fourier transform infrared spectroscopy, UV-Vis, dynamic light scattering, Photoluminescence, scanning electron microscopy, and transmission electron microscopy. The anticancer effects were assessed using MTT analysis, acridine orange/ethidium bromide, reactive oxygen species (ROS), COMET assay, annexin-V/FITC, cell cycle analysis, and expression of marker genes against HepG2 cell lines. The results showed significant cytotoxicity, apoptosis induction, and increased ROS production in treated cells compared to control cells. The nanoparticles also activated the apoptotic cascade and regulated the PI3K/AKT/mTOR pathways. The nanocomposites exhibited unique characteristics such as anticancer efficacy in vitro. Further research was conducted using zebrafish to model hematological parameters, liver enzymes, and histopathology to study effectiveness. Green-synthesized Pluronic F127-chlorogenic acid nanoparticles can be considered potential cancer therapy agents.

引用

页数：15

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