A Colorimetric Aptasensor for Ochratoxin A Detection Based on Tetramethylrhodamine Charge Effect-Assisted Silver Enhancement

被引:4
|
作者
Yang, Xiaoyan [1 ]
Huang, Rong [1 ]
Xiong, Lulu [1 ]
Chen, Feng [1 ]
Sun, Wei [2 ]
Yu, Ling [1 ]
机构
[1] Southwest Univ, Inst Clean Energy & Adv Mat, Sch Mat & Energy, Key Lab Luminescent & Real Time Analyt Chem,Minist, Chongqing 400715, Peoples R China
[2] Hainan Normal Univ, Coll Chem & Chem Engn, Haikou 571158, Peoples R China
来源
BIOSENSORS-BASEL | 2023年 / 13卷 / 04期
关键词
aptamer; ochratoxin A; gold nanoparticle; silver enhancement; colorimetric aptasensor; GOLD NANOPARTICLES; APTAMER; IMMUNOASSAY; MYCOTOXINS; B-1;
D O I
10.3390/bios13040468
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel colorimetric aptasensor based on charge effect-assisted silver enhancement was developed to detect ochratoxin A (OTA). To achieve this objective, gold nanoparticles (AuNPs), which can catalyze silver reduction and deposition, were used as the carrier of the aptamers tagged with a positively charged tetramethylrhodamine (TAMRA). Due to the mutual attraction of positive and negative charges, the TAMRA attracted and retained the silver lactate around the AuNPs. Thus, the chance of AuNP-catalyzed silver reduction was increased. The charge effect-assisted silver enhancement was verified by tagging different base pair length aptamers with TAMRA. Under optimized conditions, the as-prepared OTA aptasensor had a working range of 1 x 10(2)-1 x 10(6) pg mL(-1). The detection limit was as low as 28.18 pg mL(-1). Moreover, the proposed aptasensor has been successfully applied to determine OTA in actual samples with satisfactory results.
引用
收藏
页数:11
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