Structural Characteristic and In-Vitro Anticancer Activities of Dandelion Leaf Polysaccharides from Pressurized Hot Water Extraction

被引：14

作者：

Chen, Pei ^{[1
]}

Ding, Suyun ^{[1
]}

Yan, Zhiqian ^{[1
]}

Liu, Huiping ^{[1
]}

Tu, Jianqiu ^{[1
]}

Chen, Yi ^{[1
]}

Zhang, Xiaowei ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, State Key Lab Food Nutr & Safety, Key Lab Food Nutr & Safety,Minist Educ China, Tianjin 300457, Peoples R China

来源：

NUTRIENTS | 2023年 / 15卷 / 01期

基金：

中国国家自然科学基金;

关键词：

dandelion leaf; polysaccharides; structure; NMR; cell apoptosis; CANCER CELL-PROLIFERATION; TARAXACUM-OFFICINALE; BIOACTIVE POLYSACCHARIDES; PHYSICOCHEMICAL PROPERTIES; RHAMNOGALACTURONAN I; COMPONENTS; APOPTOSIS; PECTIN;

D O I：

10.3390/nu15010080

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Dandelion (Taraxacum mongolicum Hand.-Mazz.) is a medicinal and edible plant. Dandelion has great development value for its health promoting benefits; additionally, Dandelion grows almost anywhere in the world. In this study, we report the structural characteristics and anti-cancer activity of novel dandelion leaf polysaccharides extracted by pressurized hot water extraction at 120 degrees C (DLP120) with Mw relative to dextran of 1.64 x 10(6) Da. Structural analysis indicated that DLP120 is a complex polysaccharide composed of pectin and arabinogalactan. It was mainly composed of arabinose (32.35 mol%) and galactose (44.91 mol%). The main glycosidic linkages of DLP120 were 4-beta-D-Galp, 4-alpha-D-GalpA, T-beta-D-Galp, 5-alpha-L-Araf, 3,5-alpha-L-Araf, and T-alpha-L-Araf. In vitro, DLP120 inhibited HepG2 cell proliferation in a dose-dependent manner by inducing cell apoptosis. Cell cycle detection results revealed that DLP120 mainly arrests the cell cycle in S phase. Cells treated with DLP120 displayed obvious apoptotic morphology, including cell volume shrinks and cytoskeleton breaks down. In short, DLP120 has potential as an anti-cancer agent.

引用

页数：19

共 63 条

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