mRNA Turnover Protein 4 Is Vital for Fungal Pathogenicity and Response to Oxidative Stress in Sclerotinia sclerotiorum

被引：7
作者：
Yang, Chenghuizi [1 ,2 ]
Tang, Lan [2 ]
Qin, Lei [1 ,2 ]
Zhong, Weiping [1 ,2 ]
Tang, Xianyu [1 ,2 ]
Gong, Xin [1 ,2 ]
Xie, Wenqi [2 ]
Li, Yifu [1 ,2 ]
Xia, Shitou [1 ,2 ]
机构：
[1] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China
[2] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China
来源：
PATHOGENS | 2023年 / 12卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Sclerotinia sclerotiorum; SsMRT4; ribosome assembly; ROS; pathogenicity; RIBOSOME ASSEMBLY FACTOR; MRT4; PROTEIN; MATURATION; GENES; BIOGENESIS; EXPRESSION; MUTATIONS; SUBUNIT; ENZYMES; STALK;
D O I：
10.3390/pathogens12020281
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Ribosome assembly factors have been extensively studied in yeast, and their abnormalities may affect the assembly process of ribosomes and cause severe damage to cells. However, it is not clear whether mRNA turnover protein 4 (MRT4) functions in the fungal growth and pathogenicity in Sclerotinia sclerotiorum. Here, we identified the nucleus-located gene SsMRT4 using reverse genetics, and found that knockdown of SsMRT4 resulted in retard mycelia growth and complete loss of pathogenicity. Furthermore, mrt4 knockdown mutants showed almost no appressorium formation and oxalic acid production comparing to the wild-type and complementary strains. In addition, the abilities to ROS elimination and resistance to oxidative and osmotic stresses were also seriously compromised in mrt4 mutants. Overall, our study clarified the role of SsMRT4 in S. sclerotiorum, providing new insights into ribosome assembly in regulating pathogenicity and resistance to environmental stresses of fungi.
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页数：14
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