Side-Stream Enhanced Biological Phosphorus Removal (S2EBPR) enables effective phosphorus removal in a pilot-scale A-B stage shortcut nitrogen removal system for mainstream municipal wastewater treatment

被引：16

作者：

Wang, Dongqi ^{[1
,2
]}

Han, Il ^{[3
]}

Mccullough, Kester ^{[3
,4
]}

Klaus, Stephanie ^{[4
]}

Lee, Jangho ^{[3
]}

Srinivasan, Varun ^{[2
,5
]}

Li, Guangyu ^{[2
,3
]}

Wang, Zijian Leo ^{[6
]}

Bott, Charles B. ^{[4
]}

McQuarrie, Jim ^{[7
]}

Stinson, Beverley M. ^{[7
]}

deBarbadillo, Christine ^{[8
]}

Dombrowski, Paul ^{[8
]}

Barnard, James ^{[9
]}

Gu, April Z. ^{[2
,3
]}

机构：

[1] Xian Univ Technol, Dept Municipal & Environm Engn, Xian 710048, Shaanxi, Peoples R China

[2] Northeastern Univ, Dept Civil & Environm Engn, 360 Huntington Ave, Boston, MA 02115 USA

[3] Cornell Univ, Sch Civil & Environm Engn, 220 Hollister Hall, Ithaca, NY 14853 USA

[4] Hampton Rd,1434 Air Rail Ave, Virginia Beach, VA 23454 USA

[5] Brown & Caldwell, One Tech Dr, Andover, MA 01810 USA

[6] Cornell Univ, Coll Agr & Life Sci, Dept Biol & Environm Engn, Riley Robb Hall,106,111 Wing Dr, Ithaca, NY 14850 USA

[7] AECOM, 3275 Lakeshore Rd Su 201, Kelowna, BC V1W 3S9, Canada

[8] Woodard & Curran Inc, 1699 King St, Enfield, CT 06082 USA

[9] Black & Veatch Consulting Engineers, 8400 Ward Pkwy, Kansas City, MO 64114 USA

来源：

WATER RESEARCH | 2024年 / 251卷

关键词：

Side -stream EBPR; Shortcut N removal; Sludge fermentation; Microbial community; Phenotypic profiling; POLYPHOSPHATE-ACCUMULATING ORGANISMS; CANDIDATUS ACCUMULIBACTER; TREATMENT PLANTS; DENITRIFICATION; ANAMMOX; MICROORGANISMS; COMPETIBACTER; METABOLISM; NITRITE; MODEL;

D O I：

10.1016/j.watres.2023.121050

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

While the adsorption/bio-oxidation (A/B) process has been widely studied for carbon capture and shortcut nitrogen (N) removal, its integration with enhanced biological phosphorus (P) removal (EBPR) has been considered challenging and thus unexplored. Here, full-scale pilot testing with an integrated system combining A -stage highrate activated sludge with B -stage partial (de)nitrification/anammox and side -stream EBPR (HRAS-P(D)N/AS2EBPR) was conducted treating real municipal wastewater. The results demonstrated that, despite the relatively low influent carbon load, the B -stage P(D)N-S2EBPR system could achieve effective P removal performance, with the carbon supplement and redirection of the A -stage sludge fermentate to the S2EBPR. The novel process configuration design enabled a system shift in carbon flux and distribution for efficient EBPR, and provided unique selective factors for ecological niche partitioning among different key functionally relevant microorganisms including polyphosphate accumulating organisms (PAOs) and glycogen -accumulating organisms (GAOs). The combined nitrite from B -stage to S2EBPR and aerobic -anoxic conditions in our HRAS-P(D)N/AS2EBPR system promoted DPAOs for simultaneous internal carbon -driven denitrification via nitrite and P removal. 16S rRNA gene -based oligotyping analysis revealed high phylogenetic microdiversity within the Accumulibacter population and discovered coexistence of certain oligotypes of Accumulibacter and Competibacter correlated with efficient P removal. Single -cell Raman micro -spectroscopy -based phenotypic profiling showed high phenotypic microdiversity in the active PAO community and the involvement of unidentified PAOs and internal carbon -accumulating organisms that potentially played an important role in system performance. This is the first pilot study to demonstrate that the P(D)N-S2EBPR system could achieve shortcut N removal and influent carbon -independent EBPR simultaneously, and the results provided insights into the effects of incorporating S2EBPR into A/B process on metabolic activities, microbial ecology, and resulted system performance.

引用

页数：14