Functional annotation and comparative genomics analysis of Balamuthia mandrillaris reveals potential virulence-related genes

被引：4

作者：

Otero-Ruiz, Alejandro ^{[1
]}

Rodriguez-Anaya, Libia Zulema ^{[2
]}

Lares-Villa, Fernando ^{[3
]}

Beltran, Luis Fernando Lozano Aguirre ^{[4
]}

Lares-Jimenez, Luis Fernando ^{[3
]}

Gonzalez-Galaviz, Jose Reyes ^{[2
]}

Cruz-Mendivil, Abraham ^{[5
]}

机构：

[1] Inst Tecnol Sonora, Dept Biotecnol & Ciencias Alimentarias, Programa Doctorado Ciencias Especial Biotecnol, Obregon 85000, Sonora, Mexico

[2] CONAHCYT, Inst Tecnol Sonora, Obregon 85000, Sonora, Mexico

[3] Inst Tecnol Sonora, Dept Ciencias Agron & Vet, Obregon 85000, Sonora, Mexico

[4] Univ Nacl Autonoma Mexico, Unidad Anal Bioinformat, Ctr Ciencias Genomicas, Cuernavaca 62210, Morelos, Mexico

[5] CONAHCYT, Inst Politecn Nacl, CIIDIR Unidad Sinaloa, Guasave 81101, Sinaloa, Mexico

来源：

SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期

关键词：

GET-HOMOLOGS; SEQUENCE; VERSATILE; PLATFORM;

D O I：

10.1038/s41598-023-41657-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Balamuthia mandrillaris is a pathogenic protozoan that causes a rare but almost always fatal infection of the central nervous system and, in some cases, cutaneous lesions. Currently, the genomic data for this free-living amoeba include the description of several complete mitochondrial genomes. In contrast, two complete genomes with draft quality are available in GenBank, but none of these have a functional annotation. In the present study, the complete genome of B. mandrillaris isolated from a freshwater artificial lagoon was sequenced and assembled, obtaining an assembled genome with better assembly quality parameter values than the currently available genomes. Afterward, the genome mentioned earlier, along with strains V039 and 2046, were subjected to functional annotation. Finally, comparative genomics analysis was performed, and it was found that homologous genes in the core genome potentially involved in the virulence of Acanthamoeba spp. and Trypanosoma cruzi. Moreover, eleven of fifteen genes were identified in the three strains described as potential target genes to develop new treatment approaches for B. mandrillaris infections. These results describe proteins in this protozoan's complete genome and help prioritize which target genes could be used to develop new treatments.

引用

页数：10

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