Isolation and characterization of emerging Mpox virus from India

被引：5

作者：

Sharma, Sushil Kumar ^{[1
]}

Dash, Paban Kumar ^{[1
,4
]}

Yadav, Ram Govind ^{[1
]}

Shrivastava, Ambuj ^{[1
]}

Menon, Rohit ^{[1
]}

Kumar, Jyoti S. ^{[1
]}

Sharma, Shashi ^{[1
]}

Dhankher, Suman ^{[1
]}

Dhiman, Sunil ^{[1
]}

Kumari, Divya ^{[1
]}

Shukla, Manisha ^{[1
]}

Relhan, Vineet ^{[2
,3
]}

Kumar, Suresh ^{[2
,3
]}

Parida, Manmohan ^{[1
]}

机构：

[1] Def Res & Dev Estab DRDE, Div Virol, Gwalior, India

[2] Maulana Azad Med Coll, New Delhi, India

[3] Lok Nayak Hosp, New Delhi, India

[4] Def Res & Dev Estab DRDE, Div Virol, Jhansi Rd, Gwalior 474002, India

来源：

JOURNAL OF MEDICAL VIROLOGY | 2023年 / 95卷 / 07期

关键词：

cytopathic effect; monkeypox virus; Mpox; next gen sequencing; PCR; replication kinetics; virus isolation; MONKEYPOX VIRUS; COMMUNITY; CONGO;

D O I：

10.1002/jmv.28911

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mpox (previously known as Monkeypox) has recently re-emerged, primarily through human-to-human transmission in non-endemic countries including India. Virus isolation is still considered as the gold standard for diagnosis of viral infections. Here, the qPCR positive skin lesion sample from a patient was inoculated in Vero E6 cell monolayer. Characteristic cytopathic effect exhibiting typical cell rounding and detachment was observed at passage-02. The virus isolation was confirmed by qPCR. The replication kinetics of the isolate was determined that revealed maximum viral titre of log 6.3 PFU/mL at 72 h postinfection. Further, whole genome analysis through next generation sequencing revealed that the Mpox virus (MPXV) isolate is characterized by several unique SNPs and INDELs. Phylogenetically, it belonged to A.2 lineage of clade IIb, forming a close group with all other Indian MPXV along with few from USA, UK, Portugal, Thailand and Nigeria. This study reports the first successful isolation and phenotypic and genotypic characterization of MPXV from India.

引用

页数：14

共 39 条

[21] Minimap2: pairwise alignment for nucleotide sequences [J].

Li, Heng .

BIOINFORMATICS, 2018, 34 (18) :3094-3100

[22] The Sequence Alignment/Map format and SAMtools [J].

Li, Heng ;

Handsaker, Bob ;

Wysoker, Alec ;

Fennell, Tim ;

Ruan, Jue ;

Homer, Nils ;

Marth, Gabor ;

Abecasis, Goncalo ;

Durbin, Richard .

BIOINFORMATICS, 2009, 25 (16) :2078-2079

[23] Human Monkeypox [J].

McCollum, Andrea M. ;

Damon, Inger K. .

CLINICAL INFECTIOUS DISEASES, 2014, 58 (02) :260-267

[24] SARS-CoV-2 variant spike and accessory gene mutations alter pathogenesis [J].

McGrath, Marisa E. ;

Xue, Yong ;

Dillen, Carly ;

Oldfield, Lauren ;

Assad-Garcia, N. ;

Zaveri, Jayshree ;

Singh, Natasha ;

Baracco, Lauren ;

Taylor, Louis J. ;

Vashee, Sanjay ;

Frieman, Matthew B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2022, 119 (37)

[25]

Mendoza Emelissa J, 2020, Curr Protoc Microbiol, V57, pecpmc105, DOI [10.1002/cpmc.105, 10.1002/cpmc.105]

[26] New challenges in human monkeypox outside Africa: A review and case report from Italy [J].

Mileto, Davide ;

Riva, Agostino ;

Cutrera, Miriam ;

Moschese, Davide ;

Mancon, Alessandro ;

Meroni, Luca ;

Giacomelli, Andrea ;

Bestetti, Giovanna ;

Rizzardini, Giuliano ;

Gismondo, Maria Rita ;

Antinori, Spinello .

TRAVEL MEDICINE AND INFECTIOUS DISEASE, 2022, 49

[27]

Monkeypox, 2022, WHO INT MPOX MONK WH

[28]

Moore M, 2022, STATPEARLS

[29]

Multi-country outbreak of monkeypox, 2022, 8 MULT OUTBR MONK

[30] Reemergence of monkeypox: Prevalence, diagnostics, and countermeasures [J].

Nalca, A ;

Rimoin, AW ;

Bavari, S ;

Whitehouse, CA .

CLINICAL INFECTIOUS DISEASES, 2005, 41 (12) :1765-1771

← 1 2 3 4 →