Tracing development of song memory with fMRI in zebra finches after a second tutoring experience

Sensory experiences in early development shape higher cognitive functions such as language acquisition in humans and song learning in birds. Zebra finches (Taeniopygia guttata) sequentially exposed to two different song 'tutors' during the sensitive period in development are able to learn from their second tutor and eventually imitate aspects of his song, but the neural substrate involved in learning a second song is unknown. We used fMRI to examine neural activity associated with learning two songs sequentially. We found that acquisition of a second song changes lateralization of the auditory midbrain. Interestingly, activity in the caudolateral Nidopallium (NCL), a region adjacent to the secondary auditory cortex, was related to the fidelity of second-song imitation. These findings demonstrate that experience with a second tutor can permanently alter neural activity in brain regions involved in auditory perception and song learning. Learning song from two 'tutors' at different points in development permanently alters neural activity in auditory processing and learning regions in zebra finches.