Comparative genome analysis of the freshwater fungus Filosporella fistucella indicates potential for plant-litter degradation at cold temperatures

被引:2
作者
Vasconcelos Rissi, Daniel [1 ]
Ijaz, Maham [1 ]
Baschien, Christiane [1 ,2 ]
机构
[1] Leibniz Inst DSMZ, German Collect Microorganisms & Cell Cultures, D-38124 Braunschweig, Germany
[2] Leibniz Inst DSMZ, German Collect Microorganisms & Cell Cultures, Inhoffenstr 7B, D-38124 Braunschweig, Germany
来源
G3-GENES GENOMES GENETICS | 2023年 / 13卷 / 11期
关键词
fungal genome; freshwater fungi; long-read sequencing; comparative genomics; cold-adapt proteins; LEAF-LITTER; ENZYMATIC-ACTIVITY; SQUALENE SYNTHASE; ZARAGOZIC ACIDS; DECOMPOSITION; BIODIVERSITY; METABOLITES; ADAPTATION; PREDICTION; CELLULOSE;
D O I
10.1093/g3journal/jkad190
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Freshwater fungi play an important role in the decomposition of organic matter of leaf litter in rivers and streams. They also possess the necessary mechanisms to endure lower temperatures caused by habitat and weather variations. This includes the production of cold-active enzymes and antifreeze proteins. To better understand the physiological activities of freshwater fungi in their natural environment, different methods are being applied, and genome sequencing is one in the spotlight. In our study, we sequenced the first genome of the freshwater fungus Filosporella fistucella (45.7 Mb) and compared the genome with the evolutionary close-related species Tricladium varicosporioides (48.2 Mb). The genomes were annotated using the carbohydrate-active enzyme database where we then filtered for leaf-litter degradation-related enzymes (cellulase, hemicellulase, laccase, pectinase, cutinase, amylase, xylanase, and xyloglucanase). Those enzymes were analyzed for antifreeze properties using a machine-learning approach. We discovered that F. fistucella has more enzymes to participate in the breakdown of sugar, leaf, and wood than T. varicosporioides (855 and 719, respectively). Filosporella fistucella shows a larger set of enzymes capable of resisting cold temperatures than T. varicosporioides (75 and 66, respectively). Our findings indicate that in comparison with T. varicosporioides, F. fistucella has a greater capacity for aquatic growth, adaptability to freshwater environments, and resistance to low temperatures.
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页数:8
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