Site-selective heat boosting electrochemiluminescence for single cell imaging

被引:14
作者
Gou, Xiaodan [1 ]
Zhang, Yiwen [1 ,3 ]
Xing, Zejing [1 ]
Ma, Cheng [1 ,2 ]
Mao, Changjie [3 ]
Zhu, Jun-Jie [1 ]
机构
[1] Nanjing Univ, Sch Chem & Chem Engn, AState Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China
[2] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China
[3] Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Anhui, Peoples R China
基金
中国国家自然科学基金;
关键词
ELECTROGENERATED CHEMILUMINESCENCE; TEMPERATURE-DEPENDENCE; MICROSCOPY; SURFACE; ADHESION;
D O I
10.1039/d3sc02298f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In operando visualization of local electrochemical reactions provides mechanical insights into the dynamic transport of interfacial charge and reactant/product. Electrochemiluminescence is a crossover technique that quantitatively determines Faraday current and mass transport in a straightforward manner. However, the sensitivity is hindered by the low collision efficiency of radicals and side reactions at high voltage. Here, we report a site-selective heat boosting electrochemiluminescence microscopy. By generating a micron-scale heat point in situ at the electrode-solution interface, we achieved an enhancement of luminescence intensity up to 63 times, along with an advance of 0.2 V in applied voltage. Experimental results and finite element simulation demonstrate that the fundamental reasons are accelerated reaction rate and thermal convection via a photothermal effect. The concentrated electrochemiluminescence not only boosts the contrast of single cells by 20.54 times but also enables the site-selective cell-by-cell analysis of the heterogeneous membrane protein abundance. This electrochemical visualization method has great potential in the highly sensitive and selective analysis of local electron transfer events.
引用
收藏
页码:9074 / 9085
页数:12
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