Volumetric absorptive microsampling-LC-MS/MS assays for quantitation of giredestrant in dried human whole blood

Volumetric absorption microsampling devices offer minimally invasive and user-friendly collection of capillary blood in volumes as low as 10 mu l. Herein we describe the assay validation for determination of the selective estrogen receptor degrader giredestrant (GDC-9545) in dried human whole blood collected using the Mitra((R)) and Tasso-M20 devices. Both LC-MS/MS assays met validation acceptance criteria for the linear range 1-1000 ng/ml giredestrant. Mitra and Tasso-M20 samples were stable for 84 and 28 days at ambient conditions, respectively, and for 7-9 days at 40 and -70 degrees C. Blood hematocrit, hyperlipidemia and anticoagulant did not impact quantitation of giredestrant. These validated assays are suitable for the determination of giredestrant in dried blood samples collected using Mitra and Tasso-M20 microsampling devices. This methodology publication explores the bioanalytical considerations of the assays used to measure giredestrant in human whole blood samples collected using the Mitra and Tasso-M20 volumetric absorption microsampling devices. #Microsampling