Differential gene expression associated with flower development of mango (Mangifera indica L.) varieties with different shelf-life

被引：0

作者：

Sharma, Nimisha ^{[1
]}

Shivran, Mukesh ^{[1
]}

Singh, Narendra ^{[1
]}

Dubey, Anil Kumar ^{[1
]}

Singh, Sanjay Kumar ^{[1
]}

Sharma, Neha ^{[2
]}

Gupta, Ruchi ^{[3
]}

Vittal, Hatkari ^{[1
]}

Singh, Bikram Pratap ^{[4
]}

Sevanthi, Amitha Mithra ^{[4
]}

Singh, Nagendra Kumar ^{[4
]}

机构：

[1] ICAR Indian Agr Res Inst, Div Fruits & Hort Technol, New Delhi 110012, India

[2] IILM Acad Higher Learning, Coll Engn & Technol Greater, Noida 201310, Uttar Pradesh, India

[3] NGB Diagnost Pvt Ltd, Noida 201301, UP, India

[4] ICAR Natl Inst Plant Biotechnol, New Delhi 110012, India

来源：

GENE EXPRESSION PATTERNS | 2023年 / 47卷

关键词：

Differential gene expression; Mango; RNA sequencing; Shelf-life; Transcriptome; GENOME-WIDE IDENTIFICATION; FRUIT-DEVELOPMENT; ETHYLENE; TOOL;

D O I：

10.1016/j.gep.2022.119301

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mango (Mangifera indica L.) is one of the most important commercial fruit crop grown in many parts of the world. Major challenges affecting mango trade are short shelf-life, high susceptibility to chilling injury, post-harvest diseases and consumer demand for improved fruit quality. The objective of the present study was to reveal the key regulators present in bud and flower tissues during flower development stage, associated with fruit development and affect the shelf-life of the mango fruit. RNA-sequencing of contrasting genotypes having short and long shelf-life, was carried out. Comparative differential expression pathway studies of long shelf-life (Totapuri) and short shelf-life (Bombay Green) mango genotypes revealed a total of 177 highly differentially expressed genes. Out of 177 total genes, 101 genes from endoplasmic reticulum pathway and very few from gibberellins (3) and jasmonic acid (1) pathway were identified. Genes from endoplasmic reticulum pathway like hsp 90, SRC2, DFRA, CHS, BG3 and ASPG1 mainly up regulated in Bombay Green. Uniprotein B9R8D3 also shows up regulation in Bombay Green. Ethylene insensitive pathway gene EIL1 up regulated in Bombay Green. Gene CAD1 from phenylpropanoid pathway mainly up regulated in Bombay Green. A total of 4 SSRs and 227 SNPs were mined from these pathways specific to the shelf-life. Molecular studies of endoplasmic reticulum, phenylpropanoid, ethylene, polygalacturonase and hormone pathways at the time of bud and flower formation revealed key regulators that determine the shelf-life of mango fruit.

引用

页数：11

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