Results from the SARS-CoV-2 wastewater-based surveillance system in Denmark, July 2021 to June 2022

被引：5

作者：

Krogsgaard, Lene Wulff ^{[1
]}

Benedetti, Guido ^{[1
]}

Gudde, Aina ^{[1
]}

Richter, Stine Raith ^{[2
]}

Rasmussen, Lasse Dam ^{[2
]}

Midgley, Sofie Elisabeth ^{[2
]}

Qvesel, Amanda Gammelby ^{[2
]}

Nauta, Maarten ^{[1
]}

Bahrenscheer, Naja Stolberg ^{[1
]}

von Kappelgaard, Lene ^{[1
]}

Mcmanus, Oliver ^{[1
,3
]}

Hansen, Nicco Claudio ^{[4
]}

Pedersen, Jan Bryla ^{[5
]}

Haimes, Danny ^{[6
]}

Gamst, Jesper ^{[7
]}

Norgaard, Louise Solveig ^{[7
]}

Jorgensen, Alissa Carina Udi ^{[7
]}

Ejegod, Ditte Moller ^{[7
]}

Moller, Simone Skjodt ^{[7
]}

Clauson-Kaas, Jes ^{[8
]}

Knudsen, Ida Marie ^{[8
]}

Franck, Kristina Traeholt ^{[2
]}

Ethelberg, Steen ^{[1
,9
]}

机构：

[1] Statens Serum Inst, Dept Infect Dis Epidemiol & Prevent, Artillerivej 5, DK-2300 Copenhagen, Denmark

[2] Statens Serum Inst, Dept Virus & Microbiol Special Diagnost, Artillerivej 5, DK-2300 Copenhagen, Denmark

[3] European Ctr Dis Prevent & Control, European Programme Intervent Epidemiol Training EP, Gustav 3s Blvd 40, S-16973 Solna, Sweden

[4] Test Ctr Denmark, Statens Serum Inst, Artillerivej 5, DK-2300 Copenhagen, Denmark

[5] Statens Serum Inst, Dept Epidemiol Res, Artillerivej 5, DK-2300 Copenhagen, Denmark

[6] Danish Patient Safety Author, Isl Brygge 67, DK-2300 Copenhagen, Denmark

[7] Eurofins Environm AS, Ladelundvej 85, DK-6600 Vejen, Denmark

[8] HOFOR Greater Copenhagen Util, Orestads Blvd 35, DK-2300 Copenhagen, Denmark

[9] Univ Copenhagen, Dept Publ Hlth, Global Hlth Sect, Oster Farimagsgade 5, DK-1014 Copenhagen, Denmark

来源：

WATER RESEARCH | 2024年 / 252卷

关键词：

Wastewater-based epidemiological monitoring; SARS-CoV-2; Virus shedding; Epidemiological monitoring; Disease outbreaks; Nanopore sequencing; ENVIRONMENTAL SURVEILLANCE; RNA;

D O I：

10.1016/j.watres.2024.121223

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The microbiological analysis of wastewater samples is increasingly used for the surveillance of SARS-CoV-2 globally. We described the setup process of the national SARS-CoV-2 wastewater -based surveillance system in Denmark, presented its main results during the first year of activities, from July 2021 to June 2022, and discussed their operational significance. The Danish SARS-CoV-2 wastewater -based surveillance system was designed to cover 85 % of the population in Denmark and it entailed taking three weekly samples from 230 sites. Samples were RT-qPCR tested for SARS-CoV-2 RNA, targeting the genetic markers N1, N2 and RdRp, and for two faecal indicators, Pepper Mild Mottle Virus and crAssphage. We calculated the weekly SARS-CoV-2 RNA concentration in the wastewater from each sampling site and monitored it in view of the results from individual testing, at the national and regional levels. We attempted to use wastewater results to identify potential local outbreaks, and we sequenced positive wastewater samples using Nanopore sequencing to monitor the circulation of viral variants in Denmark. The system reached its full implementation by October 2021 and covered up to 86.4 % of the Danish population. The system allowed for monitoring of the national and regional trends of SARS-CoV2 infections in Denmark. However, the system contribution to the identification of potential local outbreaks was limited by the extensive information available from clinical testing. The sequencing of wastewater samples identified relevant variants of concern, in line with results from sequencing of human samples. Amidst the COVID-19 pandemic, Denmark implemented a nationwide SARS-CoV-2 wastewater -based surveillance system that integrated routine surveillance from individual testing. Today, while testing for COVID-19 at the community level has been discontinued, the system is on the frontline to monitor the occurrence and spread of SARS-CoV-2 in Denmark.

引用

页数：9

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