Application of Human Umbilical Cord Mesenchymal Stem Cells in Rat Spinal Cord Injury Model

被引：4

作者：

Sun, Xue-Cheng ^{[1
,2
]}

Wang, Hu ^{[1
,3
]}

Ma, Xu ^{[1
,3
,4
]}

Xia, Hong-Fei ^{[1
,3
,4
]}

机构：

[1] Natl Res Inst Family Planning, Reprod & Genet Ctr, NHC Key Lab Reprod Hlth Engn Technol Res NRIFP, Beijing, Peoples R China

[2] Zibo Maternal & Child Hlth Hosp, Med Genet, Zibo, Peoples R China

[3] Peking Union Med Coll & Chinese Acad Med Sci, Grad Sch, Beijing, Peoples R China

[4] Natl Res Inst Family Planning, Reprod & Genet Ctr, NHC Key Lab Reprod Hlth Engn Technol Res NRIFP, Beijing 10081, Peoples R China

来源：

ASAIO JOURNAL | 2023年 / 69卷 / 06期

关键词：

spinal cord injury; human umbilical cord mesenchymal stem cells; Basso; Beattie & Bresnahan Locomotor rating scale (BBB scale); rat; transplantation; SCHWANN-CELLS; FUNCTIONAL RECOVERY; TRANSPLANTATION; INTERLEUKIN-10; IMPLANTATION; IMPROVES; AXONS;

D O I：

10.1097/MAT.0000000000001938

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The treatment of spinal cord injury (SCI) is a hot topic in clinic. In this study, female rats were selected and randomly divided into four groups (normal, sham, SCI, and mesenchymal stem cells [MSCs] groups). Hemostatic forceps were used to clamp the spinal cord for 1 min to establish the SCI animal model in rats. The levels of proinflammatory factors in the blood of each group were compared 4 h after operation. The motor function of hind limb was estimated by Basso, Beattie & Bresnahan Locomotor rating scale (BBB scale) at 3 months after surgery, the spinal cord tissue from the experimental area was obtained and stained histologically and immunohistochemically. Basso, Beattie & Bresnahan Locomotor rating scale results indicated that human umbilical cord (HUC) MSCs transplantation could improve the walking ability in rats with the SCI. Human umbilical cord mesenchymal stem cells substantially upregulated the secretion of anti-inflammatory factors and downregulated the secretion of proinflammatory factors, and promoted the repair of the SCI and inhibited the increase of glial cells induced by the SCI. Human umbilical cord mesenchymal stem cells transplantation can partially recovered the motor ability of rats with the SCI through promoting the regeneration of nerve cell and the expression of neural related genes, and inhibiting inflammatory reaction.

引用

页码：E256 / E264

页数：9

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