Inhibited Expression of NLRP12 Promotes the Development of Triple-Negative Breast Cancer by Activating the NF-?B Pathway

被引:5
作者
Kuang, Wenbin [1 ]
Gu, Qingdan [2 ]
Zhou, Ying [2 ]
Xiao, Xiaoqin [3 ]
He, Dabao [1 ]
Deng, Qiuchan [1 ]
机构
[1] Guangdong Med Univ, Shenzhen Longhua Dist Cent Hosp, Dept Clin Lab, Shenzhen 518110, Peoples R China
[2] Guangdong Med Univ, Lab Med, Zhanjiang 524023, Peoples R China
[3] Guangdong Med Univ, Shenzhen Longhua Dist Cent Hosp, Dept Pathol, Shenzhen 518110, Peoples R China
关键词
NLRP12; Triple-negative breast cancer; NF-& kappa; B; Proliferation; Migration; Invasion; KAPPA-B; COLON INFLAMMATION;
D O I
10.1007/s12013-023-01166-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
NLRP12 can affect the progression of different diseases, including hepatocellular carcinoma. However, no report on triple-negative breast cancer (TNBC) has been found. Thus, this study aimed to explore the role of NLRP12 in TNBC. In our study, immunohistochemistry, real-time quantitative PCR (qPCR), and Western blot assays were used to evaluate NLRP12 expression in TNBC tissues and cells. Then, NLRP12 lentivirus was constructed and infected into MDA-MB-231 and MDA-MB-157 cells with or without PTD-p65-P1 treatment. Next, cells were collected for cell function detection using the following procedures: colony formation assay for proliferation, Transwell for migration and invasion, and Western blot for NF-?B and MAPK pathway-associated proteins. Finally, a xenograft mouse model was applied; the tumor volume and weight were determined, and NLRP12, p-I?Bb-a, and p-I?Bb-a expressions were evaluated using qPCR and Western blot. Results indicated that NLRP12 was lowly expressed in TNBC tissues and cells. The inhibition of NLRP12 could induce the proliferation, migration, and invasion of TNBC cells, which also could be reversed by inhibiting the NF-?B pathway (PTD-p65-P1). Moreover, silencing of NLRP12 could upregulate p-I?Bb-a, while I?Bb-a, p-ERK, ERK, p-p38, p38, p-JNK, and JNK expressions remained unchanged, thereby indicating that only the NF-?B pathway could be activated by NLRP12 silencing. Furthermore, the xenograft mouse model confirmed the abovementioned findings. Therefore, the low expression of NLRP12 promoted the proliferation, migration, and invasion in TNBC cells by activating the NF-?B pathway. This study might provide insights into TNBC therapy.
引用
收藏
页码:727 / 735
页数:9
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