Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment

被引:33
作者
Hashemipetroudi, Seyyed Hamidreza [1 ,2 ]
Arab, Mozhdeh [1 ,3 ]
Heidari, Parviz [4 ]
Kuhlmann, Markus [2 ]
机构
[1] Sari Agr Sci & Nat Resources Univ SANRU, Genet & Agr Biotechnol Inst Tabarestan GABIT, Dept Genet Engn & Biol, Sari, Iran
[2] Leibniz Inst Plant Genet & Crop Plant Res IPK, RG Heterosis, Gatersleben, Germany
[3] Natl Inst Genet Engn & Biotechnol NIGEB, Tehran, Iran
[4] Shahrood Univ Technol, Fac Agr, Shahrood, Iran
来源
FRONTIERS IN PLANT SCIENCE | 2023年 / 14卷
关键词
ABA treatment; salt stress; cold stress; gene expression; gene structure; plant gene family; LIGNIFICATION; PREDICTION; PROMOTER; CDNAS;
D O I
10.3389/fpls.2023.1112354
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Laccases are plant enzymes with essential functions during growth and development. These monophenoloxidases are involved in lignin polymerization, and their expression respond to environmental stress. However, studies of laccases in some plants and fungi have highlighted that many structural and functional aspects of these genes are still unknown. Here, the laccase gene family in Aeluropus littoralis (AlLAC) is described based on sequence structure and expression patterns under abiotic stresses and ABA treatment. Fifteen non-redundant AlLACs were identified from the A. littoralis genome, which showed differences in physicochemical characteristics and gene structure. Based on phylogenetic analysis, AlLACs and their orthologues were classified into five groups. A close evolutionary relationship was observed between LAC gene family members in rice and A. littoralis. According to the interaction network, AlLACs interact more with proteins involved in biological processes such as iron incorporation into the metallo-sulfur cluster, lignin catabolism, regulation of the symbiotic process and plant-type primary cell wall biogenesis. Gene expression analysis of selected AlLACs using real-time RT (reverse transcription)-PCR revealed that AlLACs are induced in response to abiotic stresses such as cold, salt, and osmotic stress, as well as ABA treatment. Moreover, AlLACs showed differential expression patterns in shoot and root tissues. Our findings indicate that AlLACs are preferentially involved in the late response of A. littoralis to abiotic stress.
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页数:12
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