Preliminary investigations of microplastic pollution in karst systems, from surface watercourses to cave waters

Microplastic pollution in different environments has increasingly been documented in detail in recent times, but it is still poorly studied in caves and karst aquifers. To deepen the knowledge of microplastic pollution, for karst environment protection and conservation purposes, we collected and investigated different water samples from a karst area of Italy, considering connected surface and cave waters. Microplastics were extracted from water samples by filtration and subjected to organic matter removal with 15% hydrogen peroxide solution. Micro -plastics on filters were counted and characterised (size, colour, shape) via visual identification under a micro-scope, with and without UV light, exploiting fluorescence given by fluorescent whitening additives contained in plastic materials. Finally, spectroscopic analyses were carried out on 10% of the microplastics observed on each filter. The concentration of microplastics in cave waters varied from 12 to 54 items/L, with a mean value of 28 items/L. In the surface water of a tributary, it was of 23 items/L, and in the downstream, it was 29 items/L. Fibres represented the most abundant shape (95.1%) in the karst system waters, and most microplastics (82.9%) were smaller than 1 mm. The majority of the microplastics were fluorescent under UV light (84.8%), and most fluorescent particles were transparent (46%). However, black microplastics (68%) were more common among the non-fluorescent ones. Polyethylene (51.7%) was the main type of microplastics found in the karst system waters. Our results show the presence of microplastics in karst systems and provide useful information for future research. Karst aquifers are open systems, subjected to possible contamination by surface pollutants. Micro -plastics in karst systems can be consumed by animals, damage ecosystems and contaminate water resources; surface karst areas and underground environments should therefore be monitored and protected, especially regarding the management of water resources. To further understand the sources and transport of microplastics within a karst system, analyses on a greater range of surface and subterranean waters throughout the world are required.