Crystal violet-based assay for the assessment of bacterial biofilm formation in medical tubing

被引：14

作者：

Borowicz, Marcin ^{[1
,2
]}

Krzyzanowska, Dorota M. ^{[2
,3
]}

Jafra, Sylwia ^{[1
,2
]}

机构：

[1] Univ Gdansk, Intercoll Fac Biotechnol, Lab Plant Microbiol, Ul Abrahama 58, PL-80307 Gdansk, Poland

[2] Univ Gdansk, Med Univ Gdansk, Ul A Abrahama 58, PL-80307 Gdansk, Poland

[3] Univ Gdansk, Intercoll Fac Biotechnol, Lab Biol Act Cpds, Ul A Abrahama 58, PL-80307 Gdansk, Poland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2023年 / 204卷

关键词：

Biofilm; Catheter; Colonization; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus; RISK-FACTORS; RESISTANCE; INFECTIONS;

D O I：

10.1016/j.mimet.2022.106656

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystal violet-based assay is widely used to evaluate the early stages of bacterial biofilm formation. Here, we modified it to study biofilm formation in medical tubing. While cost-effective and requiring no sophisticated equipment, our method provided quantitative and qualitative distinctions between the ability of four bacterial strains to colonize PVC catheters.

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页数：4

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