Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain

被引：3
作者：
Wang, Hao [1 ]
Mo, Yongfang [1 ]
Liu, Wenbo [1 ]
Niu, Chenxia [1 ]
He, Qijie [1 ]
Ren, Tongwei [1 ]
Ouyang, Kang [1 ,2 ,3 ,4 ]
Chen, Ying [1 ,2 ,3 ,4 ]
Huang, Weijian [1 ,2 ,3 ,4 ]
Wei, Zuzhang [1 ,2 ,3 ,4 ]
机构：
[1] Guangxi Univ, Coll Anim Sci & Technol, Lab Anim Infect Dis & Mol Immunol, Nanning 530005, Peoples R China
[2] Guangxi Zhuang Autonomous Reg Engn Res Ctr Vet Bio, Nanning 530004, Peoples R China
[3] Guangxi Key Lab Anim Reprod Breeding & Dis Control, Nanning 530005, Peoples R China
[4] Guangxi Coll & Univ Key Lab Prevent & Control Anim, Nanning 530005, Peoples R China
来源：
ARCHIVES OF VIROLOGY | 2024年 / 169卷 / 02期
关键词：
VESICULAR DISEASE;
D O I：
10.1007/s00705-023-05951-y
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Senecavirus A (SVA) is an emerging virus that causes vesicular disease in pigs. Construction of a full-length SVA cDNA clone is crucial for understanding its replication and pathogenesis. Here, we successfully constructed a CMV-promoter-driven infectious cDNA clone of the SVA isolate SVA/GX/CH/2018, which we named rSVA GX01. Sequence comparison between the pSVA GX01 and the parental isolate (SVA/GX/CH/2018) revealed three single-nucleotide differences. Four-week-old piglets were experimentally infected with either the parental virus or the cloned virus. The results showed that the cloned rSVA GX01 displayed weak pathogenicity in 4-week-old pigs compared to the parental virus SVA CH-GX-01-2018. The infectious clone of SVA will serve as a valuable tool for studying the viral replication cycle and for functional analysis of the viral genome.
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页数：8
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