Assessment of the Antitumor Activity of Green Biosynthesized Zinc Nanoparticles as Therapeutic Agent Against Renal Cancer in Rats

被引:11
作者
El-Sonbaty, Sawsan [1 ]
Kandil, Eman, I [2 ]
Haroun, Riham Abdel-Hamid [2 ]
机构
[1] Natl Ctr Radiat Res & Technol, Dept Radiat Microbiol, Atom Energy Author, Cairo, Egypt
[2] Ain Shams Univ, Fac Sci, Dept Biochem, Cairo, Egypt
关键词
Renal cell carcinoma; Ferric-nitrilotriacetate (Fe-NTA); Nanoparticles; Green biosynthesis; Zinc nanoparticles (Zn-NPs); White mushroom; OXIDE ZNO NANOPARTICLES; FERRIC NITRILOTRIACETATE; TOXICITY; METHYLATION; MECHANISM; APOPTOSIS; SILVER; MODEL;
D O I
10.1007/s12011-022-03126-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Zinc nanoparticles (Zn-NPs) have garnered a great deal of attention as potential cancer therapy. The use of microorganisms in the synthesis of nanoparticles emerges as an eco-friendly and exciting approach. This study was designed to assess biosynthesized Zn-NPs as therapeutic agent against kidney cancer induced by ferric-nitrilotriacetate (Fe-NTA) in rats. Zn-NPs were synthesized from edible mushroom then characterized by transmission electron microscopy analysis, dynamic light scattering, and Fourier transform infrared spectroscopy. Rats were divided into 4 different groups: group I (control), group II (Fe-NTA group), group III (Zn-NPs group), and group IV (Fe-NTA + Zn-NPs group). Animals were sacrificed then kidney and liver function tests, MDA level, glutathione, glutathione peroxidase, and superoxide dismutase activities were measured by using colorimetric methods. Caspase-3 level and carcinoembryonic antigen concentration were measured by using ELISA. Finally, DNA fragmentation was visualized by using agarose gel electrophoresis. Treatment with Zn-NPs significantly suppressed renal oxidative stress by restoring glutathione level, glutathione peroxidase, and superoxide dismutase activities and ameliorated oxidative damage parameters of lipid peroxidation as well as renal toxicity markers. Molecular and tumor markers showed significant improvement with respect to induction group, and this was well appreciated with the histopathological alteration findings in the treated groups. Microbial synthesized Zn-NPs possess antitumor-promoting activity against Fe-NTA-induced toxicity and carcinogenesis, which should be evaluated in a clinical study.
引用
收藏
页码:272 / 281
页数:10
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