Real-world EGFR testing practices for non-small-cell lung cancer by thoracic pathology laboratories across Europe

被引：18

作者：

Hofman, P. ^{[1
]}

Calabrese, F. ^{[2
]}

Kern, I. ^{[3
]}

Adam, J. ^{[4
]}

Alarcao, A. ^{[5
]}

Alborelli, I. ^{[6
]}

Anton, N. T. ^{[7
]}

Arndt, A. ^{[8
]}

Avdalyan, A. ^{[9
]}

Barberis, M. ^{[10
]}

Begueret, H. ^{[11
]}

Bisig, B. ^{[12
,13
]}

Blons, H. ^{[14
]}

Bostrom, P. ^{[15
]}

Brcic, L. ^{[16
]}

Bubanovic, G. ^{[17
,18
]}

Buisson, A. ^{[19
]}

Calio, A. ^{[20
]}

Cannone, M. ^{[21
]}

Carvalho, L. ^{[5
]}

Caumont, C. ^{[22
]}

Cayre, A. ^{[23
]}

Chalabreysse, L. ^{[24
]}

Chenard, M. P. ^{[25
]}

Conde, E. ^{[26
]}

Copin, M. C. ^{[27
]}

Cote, J. F. ^{[28
]}

D'Haene, N. ^{[29
]}

Dai, H. Y. ^{[30
]}

de Leval, L. ^{[12
,13
]}

Delongova, P. ^{[31
]}

Dencic-Fekete, M. ^{[32
]}

Fabre, A. ^{[33
]}

Ferenc, F. ^{[34
]}

Forest, F. ^{[35
]}

de Fraipont, F. ^{[36
]}

Garcia-Martos, M. ^{[37
]}

Gauchotte, G. ^{[38
]}

Geraghty, R. ^{[33
]}

Guerin, E. ^{[39
]}

Guerrero, D. ^{[40
]}

Hernandez, S. ^{[26
]}

Hurnik, P. ^{[31
]}

Jean-Jacques, B. ^{[41
]}

Kashofer, K. ^{[16
]}

Kazdal, D. ^{[42
,43
,44
]}

Lantuejoul, S. ^{[45
]}

Leonce, C. ^{[24
]}

Lupo, A. ^{[46
]}

Malapelle, U. ^{[47
]}

机构：

[1] Univ Cote Azur Nice, Lab Clin & Expt Pathol, FHU OncoAge, Louis Pasteur Hosp,IRCAN,Biobank Cote Azur BB-003, Nice, France

[2] Univ Padua, Dept CardiacThorac Vasc Sci & Publ Hlth, Padua, Italy

[3] Univ Clin Golnik, Dept Pathol, Golnik, Slovenia

[4] Grp Hosp Paris St Joseph, Dept Pathol, Paris, France

[5] Univ Coimbra, Inst Anat & Mol Pathol, IAP PM, Fac Med, Coimbra, Portugal

[6] Univ Basel, Univ Basel Hosp, Inst Med Genet & Pathol, Dept Pathol, Basel, Switzerland

[7] Paris Univ, Univ Hosp Bichat Claude Bernard, Dept Genet, Paris, France

[8] Bundeswehrkrankenhaus Ulm, Inst Pathol & Mol Pathol, Ulm, Germany

[9] Moscow Hlth Dept, Multidisciplinary Clin Ctr Kommunarka, Moscow, Russia

[10] Ist Ricovero & Cura Carattere Sci IRCCS, European Inst Oncol IEO, Oncogen Unit, Milan, Italy

[11] Hop Haut Leveque, Univ Hosp Bordeaux, Dept Pathol, Pessac, France

[12] Lausanne Univ Hosp, Dept Lab Med & Pathol, Inst Pathol, Lausanne, Switzerland

[13] Univ Lausanne, Lausanne, Switzerland

[14] Hop Europeen Georges Pompidou, Assistance Publ Hop Paris, Dept Biochem, Pharmacogen & Mol Oncol Unit, Paris, France

[15] Turku Univ Hosp, Dept Pathol, Turku, Finland

[16] Med Univ Graz, Diag & Res Inst Pathol, Graz, Austria

[17] Univ Zagreb, Dept Pathol, Lab Mol Pathol, Sch Med, Zagreb, Croatia

[18] Univ Hosp Ctr Zagreb, Zagreb, Croatia

[19] Ctr Leon Berard, Dept Biopathol, Lyon, France

[20] Univ & Hosp Trust Verona, Sect Pathol, Dept Diag & Publ Hlth, Verona, Italy

[21] Ist Ricovero & Cura Carattere Sci IRCCS, Inter Hosp Pathol Div, MultiMed, Milan, Italy

[22] Univ Hosp Bordeaux, Hosp Haut Leveque, Dept Tumor Biol, Pessac, France

[23] Jean Perrin Ctr, Dept Biopathol, Clermont Ferrand, France

[24] Groupement Hosp Est, Dept Pathol, Bron, France

[25] Univ Hosp Strasbourg, Dept Pathol, Strasbourg, France

[26] Univ Complutense Madrid, 12 Octubre Univ Hosp, Res Inst Octubre Univ Hosp, Dept Pathol,Inst 12 Octubre Univ Hosp i 12, Madrid, Spain

[27] Univ Angers, Ctr Hosp Univ Angers, Dept Pathol, Angers, France

[28] Inst Mutualiste Montsouris, Dept Pathol, Paris, France

[29] ULB, Erasme Hosp, Dept Pathol, HUB, Brussels, Belgium

[30] Trondheim Reg & Univ Hosp, St Olavs Hosp, Dept Pathol, Trondheim, Norway

[31] Univ Hosp Ostrava, Inst Mol & Clin Pathol & Med Genet, Fac Med, Ostrava, Czech Republic

[32] Med Fac, Inst Pathol, Belgrade, Serbia

[33] Univ Coll Dublin, St Vincents Univ Hosp, Dept Histopathol, Sch Med, Dublin, Ireland

[34] Univ Oradea, Dept Pathol, Oradea, Romania

[35] Univ Hosp St Etienne, Dept Pathol, St Etienne, France

[36] Grenoble Univ Hosp, Med Unit Mol Genet Hereditary Dis & Oncol, Grenoble, France

[37] Gregorio Maranon Gen Univ Hosp, Dept Pathol, Madrid, Spain

[38] CHRU Nancy, Dept Biopathol, CHRU ICL, Vandoeuvre Les Nancy, France

[39] Univ Hosp Strasbourg, Lab Biochem & Mol Biol, Dept Mol Canc Genet, Strasbourg, France

[40] Navarra Hlth Serv, Biomed Res Ctr, Pamplona, Spain

[41] CHU Caen Cote Nacre, Dept Pathol, Caen, France

[42] Heidelberg Univ Hosp, Inst Pathol, Heidelberg, Germany

[43] Translat Lung Res Ctr Heidelberg TLRC H, Heidelberg, Germany

[44] German Ctr Lung Res DZL, Heidelberg, Germany

[45] Canc Res Ctr Lyon CRCL, Ctr Leon Berard Unicanc & Pathol Res Platform, Dept Biopathol, Lyon, France

[46] Univ Paris, Hop Cochin, Assistance Publ Hop Paris, Dept Pathol, Paris, France

[47] Univ Naples Federico II, Dept Publ Hlth, Naples, Italy

[48] Thomayer Univ Hosp, Dept Pathol & Mol Med, Prague, Czech Republic

[49] Univ Lorraine, Inst Cancerol Lorraine, Dept Biopathol, Vandoeuvre Les Nancy, France

[50] Cantonal Hosp Baselland, Inst Pathol, Liestal, Switzerland

来源：

ESMO OPEN | 2023年 / 8卷 / 05期

关键词：

EGFR; survey; Europe; molecular pathology; non-small-cell lung cancer; EXPERIENCE;

D O I：

10.1016/j.esmoop.2023.101628

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Testing for epidermal growth factor receptor (EGFR) mutations is an essential recommendation in guidelines for metastatic non-squamous non-small-cell lung cancer, and is considered mandatory in European countries. However, in practice, challenges are often faced when carrying out routine biomarker testing, including access to testing, inadequate tissue samples and long turnaround times (TATs). Materials and methods: To evaluate the real-world EGFR testing practices of European pathology laboratories, an online survey was set up and validated by the Pulmonary Pathology Working Group of the European Society of Pathology and distributed to 64 expert testing laboratories. The retrospective survey focussed on laboratory organisation and daily EGFR testing practice of pathologists and molecular biologists between 2018 and 2021. Results: TATs varied greatly both between and within countries. These discrepancies may be partly due to reflex testing practices, as 20.8% of laboratories carried out EGFR testing only at the request of the clinician. Many laboratories across Europe still favour single-test sequencing as a primary method of EGFR mutation identification; 32.7% indicated that they only used targeted techniques and 45.1% used single-gene testing followed by next-generation sequencing (NGS), depending on the case. Reported testing rates were consistent over time with no significant decrease in the number of EGFR tests carried out in 2020, despite the increased pressure faced by testing facilities during the COVID-19 pandemic. ISO 15189 accreditation was reported by 42.0% of molecular biology laboratories for singletest sequencing, and by 42.3% for NGS. 92.5% of laboratories indicated they regularly participate in an external quality assessment scheme. Conclusions: These results highlight the strong heterogeneity of EGFR testing that still occurs within thoracic pathology and molecular biology laboratories across Europe. Even among expert testing facilities there is variability in testing capabilities, TAT, reflex testing practice and laboratory accreditation, stressing the need to harmonise reimbursement technologies and decision-making algorithms in Europe.

引用

页数：11

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