Decreased neutrophil counts prolong inflammation in acute pancreatitis and cause inflammation spillover to distant organs

被引：2

作者：

Fukuda, Yohei ^{[1
]}

Mori, Kosuke ^{[2
]}

Okada, Hideshi ^{[1
,3
,7
]}

Tomita, Hiroyuki ^{[2
,3
,8
]}

Suzuki, Kodai ^{[1
]}

Takada, Chihiro ^{[1
]}

Kamidani, Ryo ^{[1
]}

Kawasaki, Yuki ^{[1
]}

Fukuda, Hirotsugu ^{[1
]}

Minamiyama, Toru ^{[1
]}

Nishio, Ayane ^{[1
]}

Shimada, Takuto ^{[1
]}

Kuroda, Ayumi ^{[1
]}

Uchida, Akihiro ^{[1
]}

Kitagawa, Yuichiro ^{[1
]}

Fukuta, Tetsuya ^{[1
]}

Miyake, Takahito ^{[1
]}

Yoshida, Takahiro ^{[1
]}

Suzuki, Akio ^{[4
]}

Tetsuka, Nobuyuki ^{[5
]}

Yoshida, Shozo ^{[1
,6
]}

Ogura, Shinji ^{[1
]}

机构：

[1] Gifu Univ, Grad Sch Med, Dept Emergency & Disaster Med, Gifu, Japan

[2] Gifu Univ, Grad Sch Med, Dept Tumor Pathol, Gifu, Japan

[3] Gifu Univ, Inst Adv Study, Ctr One Med Innovat Translat Res, Gifu, Japan

[4] Gifu Univ Hosp, Dept Pharm, Gifu, Japan

[5] Gifu Univ, Grad Sch Med, Dept Infect Control, Gifu, Japan

[6] Gifu Univ, Abuse Prevent Emergency Med, Grad Sch Med, Gifu, Japan

[7] Gifu Univ, Grad Sch Med, Dept Emergency & Disaster Med, 1-1 Yanagido, Gifu 5011194, Japan

[8] Gifu Univ, Grad Sch Med, Dept Tumor Pathol, 1-1 Yanagido, Gifu 5011194, Japan

来源：

PANCREATOLOGY | 2023年 / 23卷 / 08期

关键词：

Animal study; Inflammatory response; Neutrophils; Pancreas; COLONY-STIMULATING FACTOR; CELL; GRANULOCYTE; INJURY; MICE;

D O I：

10.1016/j.pan.2023.10.020

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/objective: Acute pancreatitis is an aseptic inflammation caused by pathologically activated pancreatic enzymes and inflammatory mediators produced secondarily by neutrophils and other in-flammatory cells and is one of the most difficult diseases to treat. This study aimed to investigate the role of neutrophils in pancreatitis by examining tissue dynamics.Methods: We created a model of caerulein-induced pancreatitis in 12-week-old male granulocyte colony-stimulating factor knockout mice (G-CSF-KO) and wild-type littermate control mice (six intraperitoneal injections of caerulein [80 mg/kg body weight] at hourly intervals for 2 days). Mice were sacrificed 0, 3, 6, 12, 24, 36, 48, 72, and 168 h after caerulein administration and examined histologically.Results: The survival rate after one week of caerulein administration was 100 % in the control mice, whereas it was significantly lower (10 %) in the G-CSF-KO mice. Histological examination revealed significant hemorrhage and inflammatory cell migration in the G-CSF-KO mice, indicating prolonged inflammation. Conclusion: Prolonged inflammation was observed in the G-CSF-KO mice. Tissue cleanup by neutro-phils during the acute phase of inflammation may influence healing through the chronic phase.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of IAP and EPC.This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

引用

页码：911 / 918

页数：8

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