Mesenchymal Stromal Cell Secretome for Therapeutic Application in Skin Wound Healing: A Systematic Review of Preclinical Studies

被引:2
作者
da Silva, Maiara Marques
Olsson, Debora Cristina [2 ]
Teixeira, Bianca Luise [1 ]
Jeremias, Talita da Silva [1 ]
Trentin, Andrea Goncalves [1 ,3 ]
机构
[1] Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Florianopolis, Brazil
[2] Fed Inst Santa Catarina, Dept Vet Med, Florianopolis, Brazil
[3] Natl Inst Sci & Technol Regenerat Med, Rio De Janeiro, Brazil
关键词
Mesenchymal stem cells; Conditioned media; Secretory vesicles; Wound healing; Animal studies; STEM-CELLS; ADIPOSE-TISSUE; EXTRACELLULAR VESICLES; CONDITIONED MEDIUM; AMNIOTIC-FLUID; AGREEMENT; REPAIR;
D O I
10.1159/000526093
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Non-healing skin wounds remain a challenge in the healthcare system. In this sense, it is suggested that the secretome of mesenchymal stromal cells (MSCs) can be effective as a therapeutic strategy for regenerative medicine. Therefore, this systematic review aimed to determine the effects of treatment with a secretome derived from MSCs on the healing of skin wounds in a preclinical model of rodents (mice and rats). Studies were systematically retrieved from 6 databases and gray literature that provided 1,172 records, of which 25 met the inclusion criteria for qualitative analysis. Results revealed substantial heterogeneity among studies concerning experimental designs and methodologies, resulting in a high risk of bias. Together, the selected studies reported that treatment improved wound healing by (1) accelerating wound closure and improving skin repair quality; (2) reducing inflammation by decreasing the number of cells and inflammatory cytokines, accompanied by polarization of the M2 macrophage; (3) complete re-epithelialization and epidermal reorganization; (4) neovascularization promoted by proliferation of endothelial cells (CD34+) and increased levels of pro-angiogenic mediators; (5) better scar quality promoted by increased expression of collagen types I and III, as well as improved deposition and remodeling of collagen fibers. In conclusion, despite the need for alignment of methodological protocols and transparent reports in future studies, results show that the secretome of MSCs from different tissue sources corresponds to a promising tool of regenerative medicine for the treatment of skin wounds.
引用
收藏
页码:567 / 582
页数:16
相关论文
共 63 条
[1]   Introduction to Extracellular Vesicles: Biogenesis, RNA Cargo Selection, Content, Release, and Uptake [J].
Abels, Erik R. ;
Breakefield, Xandra O. .
CELLULAR AND MOLECULAR NEUROBIOLOGY, 2016, 36 (03) :301-312
[2]   MSC-Derived Extracellular Vesicles to Heal Diabetic Wounds: a Systematic Review and Meta-Analysis of Preclinical Animal Studies [J].
Bailey, Adrian J. M. ;
Li, Heidi ;
Kirkham, Aidan M. ;
Tieu, Alvin ;
Maganti, Harinad B. ;
Shorr, Risa ;
Fergusson, Dean A. ;
Lalu, Manoj M. ;
Elomazzen, Heidi ;
Allan, David S. .
STEM CELL REVIEWS AND REPORTS, 2022, 18 (03) :968-979
[3]  
Birch M, 2005, METH MOLEC MED, V117, P223
[4]   Mesenchymal stem cells as trophic mediators [J].
Caplan, Arnold I. ;
Dennis, James E. .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 98 (05) :1076-1084
[5]   Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing [J].
Casado-Diaz, Antonio ;
Quesada-Gomez, Jose Manuel ;
Dorado, Gabriel .
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2020, 8
[6]   Paracrine Factors of Mesenchymal Stem Cells Recruit Macrophages and Endothelial Lineage Cells and Enhance Wound Healing [J].
Chen, Liwen ;
Tredget, Edward E. ;
Wu, Philip Y. G. ;
Wu, Yaojiong .
PLOS ONE, 2008, 3 (04)
[7]   Extracellular vesicle-carried microRNA-27b derived from mesenchymal stem cells accelerates cutaneous wound healing via E3 ubiquitin ligase ITCH [J].
Cheng, Shihuan ;
Xi, Zhiyu ;
Chen, Guang ;
Liu, Kai ;
Ma, Renshi ;
Zhou, Chen .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020, 24 (19) :11254-11271
[8]  
Cochrane Collaboration, 2020, REV MAN REVMAN VERS
[9]   Therapeutic role of extracellular vesicles derived from stem cells in cutaneous wound models: A systematic review [J].
Dalirfardouei, Razieh ;
Gholoobi, Aida ;
Vahabian, Mehrangiz ;
Mahdipour, Elahe ;
Afzaljavan, Fahimeh .
LIFE SCIENCES, 2021, 273
[10]   Medical subject headings (MeSH) terms [J].
Dhammi, Ish Kumar ;
Kumar, Sudhir .
INDIAN JOURNAL OF ORTHOPAEDICS, 2014, 48 (05) :443-444