Conversion of carbon dioxide in biogas into acetic acid by Clostridium thailandense immobilized on porous support materials

This study aimed to convert CO2 in biogas into acetic acid using immobilized Clostridium thailandense cells on various support materials, including activated carbon, expanded clay, and coir. Immobilized cells and free cells were evaluated for their CO2 conversion ability into acetic acid using H(2 )as an electron donor at an H-2 to CO2 in biogas ratio of 2:1 (v/v), 30(degrees) C, 150 rpm. Results showed that immobilized cells on activated carbon increased CH(4 )content to 96.9% (v/v), and acetic acid production to 15.65 mmol/L within 96 h. These values outperformed free cells. The activated carbon -immobilized cells could be reused two times without losing efficacy in the purification of biogas and acetic acid production. This work indicates that using the immobilized cells offers a sustainable approach to biogas upgrading, reducing the environmental footprint of biogas production by increasing its energy content and purity.