Little Brown Bats (Myotis lucifugus) Support the Binding of SARS-CoV-2 Spike and Are Likely Susceptible to SARS-CoV-2 Infection

被引：2

作者：

Chothe, Shubhada K. ^{[1
,2
]}

Jakka, Padmaja ^{[1
,2
]}

Boorla, Veda Sheersh ^{[3
]}

Ramasamy, Santhamani ^{[1
,2
]}

Gontu, Abhinay ^{[1
,2
]}

Nissly, Ruth H. ^{[1
,2
]}

Brown, Justin ^{[1
,4
]}

Turner, Gregory ^{[4
]}

Sewall, Brent J. ^{[5
]}

Reeder, DeeAnn M. ^{[6
]}

Field, Kenneth A. ^{[6
]}

Engiles, Julie B. ^{[7
]}

Amirthalingam, Saranya ^{[1
]}

Ravichandran, Abirami ^{[1
]}

LaBella, Lindsey ^{[1
,2
]}

Nair, Meera Surendran ^{[1
,2
]}

Maranas, Costas D. ^{[3
]}

Kuchipudi, Suresh V. ^{[1
,2
]}

机构：

[1] Penn State Univ, Dept Vet & Biomed Sci, Anim Diagnost Lab, University Pk, PA 16802 USA

[2] Penn State Univ, Huck Inst Life Sci, Ctr Infect Dis Dynam, University Pk, PA 16802 USA

[3] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA

[4] Penn Game Commiss, 2001 Elmerton Ave, Harrisburg, PA 17110 USA

[5] Temple Univ, Dept Biol, Philadelphia, PA 19122 USA

[6] Bucknell Univ, Dept Biol, Lewisburg, PA 17837 USA

[7] Univ Penn, New Bolton Ctr, Sch Vet Med, Dept Pathobiol & Clin Studies, Kennett Sq, PA 19348 USA

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 05期

关键词：

SARS-CoV-2; ACE2; little brown bat; coronaviruses; RESPIRATORY SYNDROME CORONAVIRUS; RECEPTOR USAGE; CELL ENTRY; PROTEIN; ACE2; FERRETS; DOMAIN;

D O I：

10.3390/v15051103

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), believed to have originated from a bat species, can infect a wide range of non-human hosts. Bats are known to harbor hundreds of coronaviruses capable of spillover into human populations. Recent studies have shown a significant variation in the susceptibility among bat species to SARS-CoV-2 infection. We show that little brown bats (LBB) express angiotensin-converting enzyme 2 receptor and the transmembrane serine protease 2, which are accessible to and support SARS-CoV-2 binding. All-atom molecular dynamics (MD) simulations revealed that LBB ACE2 formed strong electrostatic interactions with the RBD similar to human and cat ACE2 proteins. In summary, LBBs, a widely distributed North American bat species, could be at risk of SARS-CoV-2 infection and potentially serve as a natural reservoir. Finally, our framework, combining in vitro and in silico methods, is a useful tool to assess the SARS-CoV-2 susceptibility of bats and other animal species.

引用

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