Expression profile and function analysis of MsCSP17 and MsCSP18 in the larval development of Mythimna separata

被引：3

作者：

Chen, Tingting ^{[1
]}

Zhou, Xue ^{[1
]}

Wu, Jing ^{[1
]}

Xing, Gaoliang ^{[1
]}

Cao, Haiqun ^{[1
]}

Huang, Yong ^{[1
]}

机构：

[1] Anhui Agr Univ, Coll Plant Protect, Anhui Prov Key Lab Crop Integrated Pest Management, Hefei, Anhui, Peoples R China

来源：

BULLETIN OF ENTOMOLOGICAL RESEARCH | 2023年 / 113卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Chemosensory protein; development; integument; Mythimna separata; RNAi; ORIENTAL ARMYWORM; IDENTIFICATION; PROTEINS; CLONING; WALKER; GENES;

D O I：

10.1017/S0007485323000354

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Chemosensory proteins (CSPs) were necessary for insect sensory system to perform important processes such as feeding, mating, spawning, and avoiding natural enemies. However, their functions in non-olfactory organs have been poorly studied. To clarify the function of CSPs in the development of Mythimna separata (Walker) larvae, two CSP genes, MsCSP17 and MsCSP18, were identified from larval integument transcriptome dataset. Both of MsCSP17 and MsCSP18 contained four conserved cysteine sites (C x (6)-C x (18)-C x (2)-C), with a signal peptide at the N-terminal. RT-qPCR analysis showed that MsCSP17 and MsCSP18 have different expression patterns among different developmental stages and tissues. MsCSP17 was highly expressed in 1st-4th instar larvae, and MsCSP18 had high expression in adults. Both genes were expressed highly in larval head, thorax, integument and mandible. Moreover, both of MsCSP17 and MsCSP18 were lowly expressed in larval integuments when larvae molted for 6 h and 9 h from 3rd to 4th instar, but highly at the beginning and end phase during molting. After injection of dsMsCSP17 and dsMsCSP18, the expression levels of two genes decreased significantly, with the body weight of larvae decreased, the mortality increased, and the eclosion rate decreased. It was suggested that MsCSP17 and MsCSP18 contributed to the development of M. separata larvae.

引用

页码：676 / 683

页数：8

共 35 条

[1] Identification of the genes involved in odorant reception and detection in the palm weevil Rhynchophorus ferrugineus, an important quarantine pest, by antennal transcriptome analysis [J].

Antony, Binu ;

Soffan, Alan ;

Jakse, Jernej ;

Abdelazim, Mahmoud M. ;

Aldosari, Saleh A. ;

Aldawood, Abdulrahman S. ;

Pain, Arnab .

BMC GENOMICS, 2016, 17

[2] SignalP 5.0 improves signal peptide predictions using deep neural networks [J].

Armenteros, Jose Juan Almagro ;

Tsirigos, Konstantinos D. ;

Sonderby, Casper Kaae ;

Petersen, Thomas Nordahl ;

Winther, Ole ;

Brunak, Soren ;

von Heijne, Gunnar ;

Nielsen, Henrik .

NATURE BIOTECHNOLOGY, 2019, 37 (04) :420-+

[3] Identification of Candidate Olfactory Genes in Chilo suppressalis by Antennal Transcriptome Analysis [J].

Cao, Depan ;

Liu, Yang ;

Wei, Jinjin ;

Liao, Xinyan ;

Walker, William B. ;

Li, Jianhong ;

Wang, Guirong .

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2014, 10 (08) :846-860

[4] Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta [J].

Cheng, Daifeng ;

Lu, Yongyue ;

Zeng, Ling ;

Liang, Guangwen ;

He, Xiaofang .

SCIENTIFIC REPORTS, 2015, 5

[5] ApE, A Plasmid Editor: A Freely Available DNA Manipulation and Visualization Program [J].

Davis, M. Wayne ;

Jorgensen, Erik M. .

FRONTIERS IN BIOINFORMATICS, 2022, 2

[6] Transcriptome analysis of molecular mechanisms responsible for light-stress response in Mythimna separata (Walker) [J].

Duan, Yun ;

Gong, ZhongJun ;

Wu, RenHai ;

Miao, Jin ;

Jiang, YueLi ;

Li, Tong ;

Wu, XiaoBo ;

Wu, YuQing .

SCIENTIFIC REPORTS, 2017, 7

[7]

Gasteiger E., 2005, The Proteomics Protocols Handbook, P571, DOI [10.1385/1-59259-890-0:571, DOI 10.1385/1-59259-890-0:571, 10.1385/1-59259-584-7:531]

[8] Cloning and characterization of three chemosensory proteins from Spodoptera exigua and effects of gene silencing on female survival and reproduction [J].