Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform

被引：26

作者：

Cao, Ruijie ^{[1
,2
]}

Li, Yaning ^{[1
,2
]}

Chen, Xin ^{[1
,2
]}

Ge, Xichuan ^{[3
]}

Li, Meiqi ^{[1
,2
]}

Guan, Meiling ^{[1
,2
]}

Hou, Yiwei ^{[1
,2
]}

Fu, Yunzhe ^{[1
,2
]}

Xu, Xinzhu ^{[1
,2
]}

Jiang, Shan ^{[4
]}

Gao, Baoxiang ^{[3
]}

Xi, Peng ^{[1
,2
]}

机构：

[1] Peking Univ, Coll Future Technol, Dept Biomed Engn, Beijing, Peoples R China

[2] Peking Univ, Natl Biomed Imaging Ctr, Beijing, Peoples R China

[3] Hebei Univ, Coll Chem & Environm Sci, Key Lab Analyt Sci & Technol Hebei Prov, Baoding, Peoples R China

[4] Beijing Inst Collaborat Innovat, Inst Biomed Engn, Beijing, Peoples R China

来源：

NATURE METHODS | 2023年 / 20卷 / 08期

基金：

中国国家自然科学基金;

关键词：

CELLS; LIMIT;

D O I：

10.1038/s41592-023-01958-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Open-3DSIM is a versatile open-source software for high-fidelity reconstruction of three-dimensional structured illumination microscopy data (with polarization). It is available in three convenient forms for user-friendly and customizable applications. Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise levels. Open-3DSIM also offers the capacity to extract dipole orientation, paving a new avenue for interpreting subcellular structures in six dimensions (xyz & theta;& lambda;t). The platform is available as MATLAB code, a Fiji plugin and an Exe application to maximize user-friendliness.

引用

页码：1183 / +

页数：16

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