Conformational transitions of the HIV-1 Gag polyprotein upon multimerization and gRNA binding

被引:4
作者
Banerjee, Puja [1 ,2 ]
Voth, Gregory A. [1 ,2 ]
机构
[1] Univ Chicago, Inst Biophys Dynam, Chicago Ctr Theoret Chem, Dept Chem, Chicago, IL 60637 USA
[2] Univ Chicago, James Franck Inst, Chicago, IL 60637 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
MOLECULAR-DYNAMICS; NUCLEOCAPSID PROTEIN; MEMBRANE-BINDING; SOFTWARE NEWS; ZINC FINGERS; MATRIX; RNA; ASSOCIATION; P6; SIMULATIONS;
D O I
10.1016/j.bpj.2023.11.017
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
During the HIV-1 assembly process, the Gag polyprotein multimerizes at the producer cell plasma membrane, resulting in the formation of spherical immature virus particles. Gag-genomic RNA (gRNA) interactions play a crucial role in the multimerization process, which is yet to be fully understood. We performed large-scale all-atom molecular dynamics simulations of membrane-bound full-length Gag dimer, hexamer, and 18-mer. The inter-domain dynamic correlation of Gag, quantified by the heterogeneous elastic network model applied to the simulated trajectories, is observed to be altered by implicit gRNA binding, as well as the multimerization state of the Gag. The lateral dynamics of our simulated membrane-bound Gag proteins, with and without gRNA binding, agree with prior experimental data and help to validate our simulation models and methods. The gRNA binding is observed to affect mainly the SP1 domain of the 18-mer and the matrix-capsid linker domain of the hexamer. In the absence of gRNA binding, the independent dynamical motion of the nucleocapsid domain results in a collapsed state of the dimeric Gag. Unlike stable SP1 helices in the six-helix bundle, without IP6 binding, the SP1 domain undergoes a spontaneous helix-to-coil transition in the dimeric Gag. Together, our findings reveal conformational switches of Gag at different stages of the multimerization process and predict that the gRNA binding reinforces an efficient binding surface of Gag for multimerization, and also regulates the dynamic organization of the local membrane region itself.
引用
收藏
页码:42 / 56
页数:15
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