Current commercial dPCR platforms: technology and market review

被引：57

作者：

Tan, Li Ling ^{[1
,2
]}

Loganathan, Nitin ^{[1
]}

Agarwalla, Sushama ^{[3
]}

Yang, Chun ^{[4
]}

Yuan, Weiyong ^{[5
,6
,7
]}

Zeng, Jasmine ^{[1
]}

Wu, Ruige ^{[1
]}

Wang, Wei ^{[1
]}

Duraiswamy, Suhanya ^{[3
]}

机构：

[1] Singapore Inst Mfg Technol, Singapore, Singapore

[2] Nanyang Technol Univ, Mat Sci & Engn Sch, Singapore, Singapore

[3] Indian Inst Technol Hyderabad, Dept Chem Engn, Hyderabad, India

[4] Nanyang Technol Univ, Mech & Aerosp Engn Sch, Singapore, Singapore

[5] Southwest Univ, Inst Clean Energy & Adv Mat, Fac Mat & Energy, Chongqing, Peoples R China

[6] Chongqing Key Lab Adv Mat & Technol Clean Energie, Chongqing, Peoples R China

[7] Zhejiang Univ, Ningbo Res Inst, Ningbo, Peoples R China

来源：

CRITICAL REVIEWS IN BIOTECHNOLOGY | 2023年 / 43卷 / 03期

关键词：

Digital PCR; polymerase chain reaction; equipment; instrument; real-time PCR; QX100; QX200; QuantStudio; DROPLET DIGITAL PCR; REAL-TIME PCR; CIRCULATING TUMOR DNA; GENETICALLY-ENGINEERED TRAITS; CELL LUNG-CANCER; ABSOLUTE QUANTIFICATION; COPY NUMBER; QUANTITATIVE DETECTION; EGFR T790M; NONINVASIVE DETECTION;

D O I：

10.1080/07388551.2022.2037503

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Digital polymerase chain reaction (dPCR) technology has provided a new technique for molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and specificity over quantitative real-time PCRs (qPCR). Eight companies have offered commercial dPCR instruments: Fluidigm Corporation, Bio-Rad, RainDance Technologies, Life Technologies, Qiagen, JN MedSys Clarity, Optolane, and Stilla Technologies Naica. This paper discusses the working principle of each offered dPCR device and compares the associated: technical aspects, usability, costs, and current applications of each dPCR device. Lastly, up-and-coming dPCR technologies are also presented, as anticipation of how the dPCR device landscape may likely morph in the next few years.

引用

页码：433 / 464

页数：32

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