Current commercial dPCR platforms: technology and market review

被引：59

作者：

Tan, Li Ling ^{[1
,2
]}

Loganathan, Nitin ^{[1
]}

Agarwalla, Sushama ^{[3
]}

Yang, Chun ^{[4
]}

Yuan, Weiyong ^{[5
,6
,7
]}

Zeng, Jasmine ^{[1
]}

Wu, Ruige ^{[1
]}

Wang, Wei ^{[1
]}

Duraiswamy, Suhanya ^{[3
]}

机构：

[1] Singapore Inst Mfg Technol, Singapore, Singapore

[2] Nanyang Technol Univ, Mat Sci & Engn Sch, Singapore, Singapore

[3] Indian Inst Technol Hyderabad, Dept Chem Engn, Hyderabad, India

[4] Nanyang Technol Univ, Mech & Aerosp Engn Sch, Singapore, Singapore

[5] Southwest Univ, Inst Clean Energy & Adv Mat, Fac Mat & Energy, Chongqing, Peoples R China

[6] Chongqing Key Lab Adv Mat & Technol Clean Energie, Chongqing, Peoples R China

[7] Zhejiang Univ, Ningbo Res Inst, Ningbo, Peoples R China

来源：

CRITICAL REVIEWS IN BIOTECHNOLOGY | 2023年 / 43卷 / 03期

关键词：

Digital PCR; polymerase chain reaction; equipment; instrument; real-time PCR; QX100; QX200; QuantStudio; DROPLET DIGITAL PCR; REAL-TIME PCR; CIRCULATING TUMOR DNA; GENETICALLY-ENGINEERED TRAITS; CELL LUNG-CANCER; ABSOLUTE QUANTIFICATION; COPY NUMBER; QUANTITATIVE DETECTION; EGFR T790M; NONINVASIVE DETECTION;

D O I：

10.1080/07388551.2022.2037503

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Digital polymerase chain reaction (dPCR) technology has provided a new technique for molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and specificity over quantitative real-time PCRs (qPCR). Eight companies have offered commercial dPCR instruments: Fluidigm Corporation, Bio-Rad, RainDance Technologies, Life Technologies, Qiagen, JN MedSys Clarity, Optolane, and Stilla Technologies Naica. This paper discusses the working principle of each offered dPCR device and compares the associated: technical aspects, usability, costs, and current applications of each dPCR device. Lastly, up-and-coming dPCR technologies are also presented, as anticipation of how the dPCR device landscape may likely morph in the next few years.

引用

页码：433 / 464

页数：32

共 265 条

[1] Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing [J].

Aigrain, Louise ;

Gu, Yong ;

Quail, Michael A. .

BMC GENOMICS, 2016, 17

[2] Performance of a real-time PCR-based approach and droplet digital PCR in detecting human parechovirus type 3 RNA [J].

Aizawa, Yuta ;

Koyama, Akihide ;

Ishihara, Tomohiko ;

Onodera, Osamu ;

Saitoh, Akihiko .

JOURNAL OF CLINICAL VIROLOGY, 2016, 84 :27-31

[3] RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia [J].

Alikian, Mary ;

Whale, Alexandra S. ;

Akiki, Susanna ;

Piechocki, Kim ;

Torrado, Celia ;

Myint, Thet ;

Cowen, Simon ;

Griffiths, Michael ;

Reid, Alistair G. ;

Apperley, Jane ;

White, Helen ;

Huggett, Jim F. ;

Foroni, Letizia .

CLINICAL CHEMISTRY, 2017, 63 (02) :525-531

[4] Formation of dispersions using "flow focusing" in microchannels [J].

Anna, SL ;

Bontoux, N ;

Stone, HA .

APPLIED PHYSICS LETTERS, 2003, 82 (03) :364-366

[5]

[Anonymous], INTRO NGS

[6]

[Anonymous], JN MEDSYS INTRO NEW

[7]

[Anonymous], 2019, GLOBAL DIGITAL PCR M

[8]

[Anonymous], NAICAR SYSTEM CRYSTA

[9]

[Anonymous], 2021, FLUIDIGM HIGH DEFINI

[10]

[Anonymous], 2020, OPT KAIR 2019 NCOV D

← 1 2 3 4 5 6 7 8 9 10 →