DNA barcode identification of cultivated and wild tropical fruit species

被引:2
作者
Tnah, Lee Hong [1 ]
Lee, Soon Leong [1 ]
Lee, Chai Ting [1 ]
Ng, Kevin Kit Siong [1 ]
Ng, Chin Hong [1 ]
Zawiah, Ngah [1 ]
机构
[1] Forest Res Inst Malaysia, Kepong 52109, Selangor Darul, Malaysia
关键词
DNA barcoding; Chloroplast DNA; Species identification; rbcL; ITS2; trnH-psbA; INTERNAL TRANSCRIBED SPACER; LAND PLANTS; JUICES; RBCL;
D O I
10.1007/s13205-023-03848-w
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
With the rapid growth of the fruit industry worldwide, it is important to assess adulteration to ensure the authenticity and the safety of fruit products. The DNA barcoding approach offers a quick and accurate way of identifying and authenticating species. In this study, we developed reference DNA barcodes (rbcL, ITS2, and trnH-psbA) for 70 cultivated and wild tropical fruit species, representing 43 genera and 26 families. In terms of species recoverability, rbcL has a greater recoverability (100%) than ITS2 (95.7%) and trnH-psbA (88.6%). We evaluated the performance of these barcodes in species discrimination using similarity BLAST, phylogenetic tree, and barcoding gap analyses. The efficiency of rbcL, ITS2, and trnH-psbA in discriminating species was 80%, 100%, and 93.6%, respectively. We employed a multigene-tiered approach for species identification, with the rbcL region used for primary differentiation and ITS2 or trnH-psbA used for secondary differentiation. The two-locus barcodes rbcL + ITS2 and rbcL + trnH-psbA demonstrated robustness, achieving species discrimination rates of 100% and 94.3% respectively. Beyond the conventional species identification method based on plant morphology, the developed reference barcodes will aid the fruit agroindustry and trade, by making fruit-based product authentication possible.
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页数:10
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