Generation of 3D ex vivo mouse- and patient-derived glioma explant slice model for integration of confocal time-lapse imaging and spatial analysis

被引：1
|
作者：
Comba, Andre [1 ,2 ,3 ]
Varela, Maria Luisa [1 ,2 ,3 ]
Faisal, Syed M. [1 ,2 ,3 ]
Abel II, Clifford C. [1 ,2 ,3 ]
Argento, Anna E. [1 ,3 ,4 ]
Al-Holou, Wajd N. [1 ]
Hollon, Todd C. [1 ]
Perelman, Jacqueline D. [1 ,2 ]
Dunn, Patrick J. [1 ,2 ,3 ]
Motsch, Sebastien [5 ]
Castro, Maria G. [1 ,2 ,3 ]
Lowenstein, Pedro R. [1 ,2 ,3 ,4 ]
机构：
[1] Univ Michigan, Med Sch, Dept Neurosurg, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Med Sch, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Med Sch, Rogel Canc Ctr, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[5] Arizona State Univ, Sch Math & Stat Sci, Tempe, AZ USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Biotechnology and Bioengineering; Cancer; Cell Biology; Cell-based Assays; Health Sciences; Microscopy; Model Organisms;
D O I：
10.1016/j.xpro.2023.102174
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Development of spatial-integrative pre-clinical models is needed for glioblastoma, which are heterogenous tumors with poor prognosis. Here, we present an optimized protocol to generate three-dimensional ex vivo explant slice glioma model from orthotopic tumors, genetically engineered mouse models, and fresh patient-derived specimens. We describe a step-by-step workflow for tissue acquisition, dissection, and sectioning of 300 -mm tumor slices maintaining cell viability. The explant slice model allows the integration of confocal timelapse imaging with spatial analysis for studying migration, invasion, and tumor microenvironment, making it a valuable platform for testing effective treatment modalities.For complete details on the use and execution of this protocol, please refer to Comba et al. (2022).1
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页数：28
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