Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels

被引:10
作者
Sasaki, Takema [1 ]
Saito, Kei [2 ,3 ]
Inoue, Daisuke [4 ]
Serk, Henrik [5 ]
Sugiyama, Yuki [1 ,6 ]
Pesquet, Edouard [5 ,7 ]
Shimamoto, Yuta [2 ,3 ]
Oda, Yoshihisa [1 ]
机构
[1] Nagoya Univ, Grad Sch Sci, Dept Biol Sci, Furo Cho,Chikusa Ku, Nagoya, Aichi, Japan
[2] Natl Inst Genet, Dept Chromosome Sci, Mishima, Shizuoka, Japan
[3] SOKENDAI Univ, Dept Genet, Mishima, Shizuoka, Japan
[4] Kyushu Univ, Fac Design, Fukuoka, Japan
[5] Umea Univ, Umea Plant Sci Ctr UPSC, Dept Plant Physiol, Umea, Sweden
[6] Nagoya Univ, Inst Adv Res, Furo Cho,Chikusa Ku, Nagoya, Aichi, Japan
[7] Stockholm Univ, Dept Ecol Environm & Plant Sci DEEP, Arrhenius Labs, Stockholm, Sweden
关键词
GATEWAY BINARY VECTORS; PROTEIN; GENE; RHO; VISUALIZATION; RESISTANCE; NICKASES; DRIVEN; MOTION;
D O I
10.1038/s41467-023-42487-w
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Properly patterned deposition of cell wall polymers is prerequisite for the morphogenesis of plant cells. A cortical microtubule array guides the two-dimensional pattern of cell wall deposition. Yet, the mechanism underlying the three-dimensional patterning of cell wall deposition is poorly understood. In metaxylem vessels, cell wall arches are formed over numerous pit membranes, forming highly organized three-dimensional cell wall structures. Here, we show that the microtubule-associated proteins, MAP70-5 and MAP70-1, regulate arch development. The map70-1 map70-5 plants formed oblique arches in an abnormal orientation in pits. Microtubules fit the aperture of developing arches in wild-type cells, whereas microtubules in map70-1 map70-5 cells extended over the boundaries of pit arches. MAP70 caused the bending and bundling of microtubules. These results suggest that MAP70 confines microtubules within the pit apertures by altering the physical properties of microtubules, thereby directing the growth of pit arches in the proper orientation. This study provides clues to understanding how plants develop three-dimensional structure of cell walls. In plant metaxylem, three-dimensional cell wall arches are formed over pit membranes. Here, the authors show that the microtubule-associated proteins, MAP70-5 and MAP70-1, confine microtubules within the pit aperture and direct growth of pit arches in the proper orientation.
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页数:14
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