A Library of Elastin-like Proteins with Tunable Matrix Ligands for In Vitro 3D Neural Cell Culture
被引:4
|
作者:
Suhar, Riley A.
论文数: 0引用数: 0
h-index: 0
机构:
Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USAStanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
Suhar, Riley A.
[1
]
Huang, Michelle S.
论文数: 0引用数: 0
h-index: 0
机构:
Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA
Stanford Univ, Inst Chem, Engn & Med Human Hlth Sarafan ChEM H, Stanford, CA 94305 USAStanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
Huang, Michelle S.
[2
,3
]
Navarro, Renato S.
论文数: 0引用数: 0
h-index: 0
机构:
Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USAStanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
Navarro, Renato S.
[1
]
Aviles Rodriguez, Giselle
论文数: 0引用数: 0
h-index: 0
机构:
Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USAStanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
Aviles Rodriguez, Giselle
[1
]
Heilshorn, Sarah C.
论文数: 0引用数: 0
h-index: 0
机构:
Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USAStanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
Heilshorn, Sarah C.
[1
]
机构:
[1] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA
[3] Stanford Univ, Inst Chem, Engn & Med Human Hlth Sarafan ChEM H, Stanford, CA 94305 USA
Hydrogels with encapsulated cells have widespread biomedical applications, both as tissue-mimetic 3D cultures in vitro and as tissue-engineered therapies in vivo. Within these hydrogels, the presentation of cell-instructive extracellular matrix (ECM)-derived ligands and matrix stiffness are critical factors known to influence numerous cell behaviors. While individual ECM biopolymers can be blended together to alter the presentation of cell-instructive ligands, this typically results in hydrogels with a range of mechanical properties. Synthetic systems that allow for the facile incorporation and modulation of multiple ligands without modification of matrix mechanics are highly desirable. In the present work, we leverage protein engineering to design a family of xeno-free hydrogels (i.e., devoid of animal-derived components) consisting of recombinant hyaluronan and recombinant elastin-like proteins (ELPs), cross-linked together with dynamic covalent bonds. The ELP components incorporate cell-instructive peptide ligands derived from ECM proteins, including fibronectin (RGD), laminin (IKVAV and YIGSR), collagen (DGEA), and tenascin-C (PLAEIDGIELTY and VFDNFVL). By carefully designing the protein primary sequence, we form 3D hydrogels with defined and tunable concentrations of cell-instructive ligands that have similar matrix mechanics. Utilizing this system, we demonstrate that neurite outgrowth from encapsulated embryonic dorsal root ganglion (DRG) cultures is significantly modified by cell-instructive ligand content. Thus, this library of protein-engineered hydrogels is a cell-compatible system to systematically study cell responses to matrix-derived ligands.
机构:
Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USAVirginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USA
Link, Patrick A.
Pouliot, Robert A.
论文数: 0引用数: 0
h-index: 0
机构:
Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USAVirginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USA
Pouliot, Robert A.
Mikhaiel, Nabil S.
论文数: 0引用数: 0
h-index: 0
机构:
Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USAVirginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USA
Mikhaiel, Nabil S.
Young, Bethany M.
论文数: 0引用数: 0
h-index: 0
机构:
Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USAVirginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USA
Young, Bethany M.
Heise, Rebecca L.
论文数: 0引用数: 0
h-index: 0
机构:
Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USA
Virginia Commonwealth Univ, Dept Physiol & Biophys, Richmond, VA 23284 USAVirginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USA
Heise, Rebecca L.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,
2017,
(119):
机构:
Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USAUniv Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA
Diekjurgen, Dorina
Grainger, David W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA
Univ Utah, Dept Bioengn, Salt Lake City, UT 84112 USAUniv Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA
机构:
Univ Naples Federico II, Interdisciplinary Res Ctr Biomat CRIB, Naples, Italy
Univ Naples Federico II, Dept Chem Mat & Ind Prod DICMAPI, Naples, Italy
Ist Italiano Tecnol, Ctr Adv Biomat HealthCare CRIB, Naples, ItalyUniv Naples Federico II, Interdisciplinary Res Ctr Biomat CRIB, Naples, Italy
Urciuolo, F.
Imparato, G.
论文数: 0引用数: 0
h-index: 0
机构:
Ist Italiano Tecnol, Ctr Adv Biomat HealthCare CRIB, Naples, ItalyUniv Naples Federico II, Interdisciplinary Res Ctr Biomat CRIB, Naples, Italy
Imparato, G.
Netti, P. A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Naples Federico II, Interdisciplinary Res Ctr Biomat CRIB, Naples, Italy
Univ Naples Federico II, Dept Chem Mat & Ind Prod DICMAPI, Naples, Italy
Ist Italiano Tecnol, Ctr Adv Biomat HealthCare CRIB, Naples, ItalyUniv Naples Federico II, Interdisciplinary Res Ctr Biomat CRIB, Naples, Italy
机构:
Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USAUniv Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USA
Tibbitt, Mark W.
Anseth, Kristi S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USA
Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USAUniv Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USA